Kerro Dego Oudessa, Pacha Paulina A, Gillespie Barbara E, Pighetti Gina M
Department of Animal Science, Knoxville, The University of Tennessee, TN 37996, USA.
Facultad de Ciencias Veterinarias, Universidad de Concepción, Chillan 370000, Chile.
Animals (Basel). 2020 Apr 25;10(5):751. doi: 10.3390/ani10050751.
Mastitis is inflammation of mammary glands usually caused by bacteria such as . Dairy cows are susceptible to mastitis during early dry and transition periods. Effective vaccine is needed during these periods. One of the limitations to develop an effective vaccine against is the absence of good infection model. Intramammary infusion (IMIF) with has been used as an infection model to test vaccine efficacy. IMIF is reliable in causing mastitis, but it bypasses physical barriers, non-specific natural defenses, and immunity in the teat canal. IMIF also transfers a large number of bacteria into the intramammary area at once. The objective of this study was to develop IMIF model that mimics natural infection. Eight Holstein dairy cows were randomly divided into two groups of experimental ( = 5) and control ( = 3) cows. All teats of experimental cows were dipped in culture suspension, whereas that of control cows were dipped in phosphate-buffered saline. Results showed that four of five cows were infected with challenge strain by day 3 of the challenge. The remaining cow was infected with In conclusion, an experimental intramammary infection can be induced by teat dipping into bacterial suspension.
乳腺炎是乳腺的炎症，通常由诸如……等细菌引起。奶牛在干奶初期和围产期易患乳腺炎。在这些时期需要有效的疫苗。开发针对……的有效疫苗的局限性之一是缺乏良好的感染模型。用……进行乳房内灌注（IMIF）已被用作测试疫苗效力的感染模型。IMIF在引发乳腺炎方面是可靠的，但它绕过了物理屏障、非特异性天然防御以及乳头管中的免疫。IMIF还会一次性将大量细菌转移到乳房内区域。本研究的目的是开发一种模拟自然感染的IMIF模型。八头荷斯坦奶牛被随机分为两组，实验组（n = 5）和对照组（n = 3）奶牛。实验组奶牛的所有乳头都浸在……培养悬液中，而对照组奶牛的乳头浸在磷酸盐缓冲盐水中。结果显示，在攻毒第3天时，五头奶牛中有四头被攻毒株感染。其余一头奶牛被……感染。总之，通过将乳头浸入细菌悬液可诱导实验性乳房内感染。
