Key Laboratory of Storage and Processing of Plant Agro-Products, College of Biological Science and Engineering, North Minzu University, Yinchuan, Ningxia, China; Physical and Chemical Department, Ningxia Hui Autonomous Region Center for Disease Control and Prevention, Yinchuan, Ningxia, China.
National Institute of Occupational Health and Poison Control, Chinese Centre for Disease Control and Prevention, Beijing, China.
Toxicon. 2020 May;179:72-75. doi: 10.1016/j.toxicon.2020.03.003. Epub 2020 Mar 16.
Mushroom poisoning is a serious food safety issue in China. However, there is insufficient information on many poisoning incidents, including mushroom species and their clinical manifestations, diagnosis, treatments and toxins. Detailed epidemiological investigation was conducted after the occurrence of a mushroom poisoning incident resulting in typical muscarinic syndrome in Ningxia, China. The suspected mushroom species was identified based on morphological and phylogenetic analyses. Muscarine was detected using ultrahigh-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). On September 2, 2019, two patients exhibited typical muscarinic syndrome after consuming wild mushrooms. The clinical manifestations included chills, sweating, salivation and diarrhoea; the incubation period was approximately 2 h. Treatments, including anti-inflammatory, detoxification and nutritional support, were remedial. Full recovery ensued within 24 h. The specimen was identified as Inocybe serotina, and its muscarine content was 324.0 ± 62.4 mg/kg (k = 2, p = 95%). Two patients were poisoned via stimulation of their parasympathetic nervous system due to mistaken consumption of muscarine-containing I. serotina. They fully recovered with supportive treatments. To our knowledge, this is the first case report of I. serotina poisoning worldwide and is the first record of this species in China. Further, a method for muscarine detection was established using UPLC-MS/MS.
在中国，蘑菇中毒是一个严重的食品安全问题。然而，许多中毒事件的信息都不充分，包括蘑菇种类及其临床表现、诊断、治疗和毒素。在中国宁夏发生了一起蘑菇中毒事件，导致典型的毒蕈碱综合征后，进行了详细的流行病学调查。根据形态学和系统发育分析，确定了疑似蘑菇物种。使用超高效液相色谱串联质谱法（UPLC-MS/MS）检测毒蕈碱。2019 年 9 月 2 日，两名患者在食用野生蘑菇后出现典型的毒蕈碱综合征。临床表现包括寒战、出汗、流涎和腹泻；潜伏期约 2 小时。采用抗炎、解毒和营养支持等治疗方法进行补救。24 小时内完全恢复。标本被鉴定为丝盖伞属的丝盖伞，其毒蕈碱含量为 324.0±62.4mg/kg（k=2，p=95%）。两名患者因误食用含有毒蕈碱的丝盖伞属而导致副交感神经系统受到刺激而中毒。他们通过支持性治疗完全康复。据我们所知，这是全球首例丝盖伞属中毒的病例报告，也是该物种在中国的首例记录。此外，还建立了一种使用 UPLC-MS/MS 检测毒蕈碱的方法。
