Erdemli Zeynep, Erdemli Mehmet Erman, Turkoz Yusuf, Yigitcan Birgul, Aladag Mehmet Arif, Cigremis Yilmaz, Cırık Rumeyza Hilal, Altinoz Eyup, Bag Harika Gozukara
Department of Medical Biochemistry, Medical Faculty, Inonu University , Malatya, Turkey.
Department of Histology and Embryology, Medical Faculty, Inonu University , Malatya, Turkey.
Biotech Histochem. 2021 Jan;96(1):11-19. doi: 10.1080/10520295.2020.1751880. Epub 2020 Apr 29.
We investigated the effects of acrylamide (AA) and vitamin E treatment during pregnancy on brain tissues of fetuses and on adult rats. Pregnant rats were divided into five groups: control, corn oil, vitamin E, AA, vitamin E +AA. The rats administered AA received10 mg/kg/day and those administered vitamin E received 100 mg/kg/day both by via oral gavage for 20 days. On day 20 of pregnancy, half of the pregnant rats were removed by cesarean section in each group. Morphological development parameters were measured in each fetus and histopathological, biochemical and genetic analyses were conducted on the fetuses. The remaining pregnant rats in each group gave birth to the fetuses vaginally and biochemical, histopathological, genetic and cognitive function tests were conducted when the pups were 8 weeks old. AA administration caused adverse effects on fetus number, fetal weight, crown-rump length, placenta and brain weight. AA negatively affected malondialdehyde, reduced glutathione, total oxidant and antioxidant status, brain derived neurotrophic factor (BDNF) levels, brain tissue morphology, histopathology error score and gene expression (BDNF/β-actin mRNA ratio) in fetuses. AA administration caused disruption of biochemical, histopathological and cognitive functions in adult rats. Vitamin E provided protection against neurotoxicity in both fetuses and adult rats. We conclude that exposure to AA during pregnancy should be avoided and adequate amounts of antioxidants, such as vitamin E, should be consumed.
我们研究了孕期丙烯酰胺（AA）和维生素E处理对胎儿脑组织及成年大鼠的影响。将怀孕大鼠分为五组：对照组、玉米油组、维生素E组、AA组、维生素E + AA组。给予AA的大鼠通过灌胃给予10 mg/kg/天，给予维生素E的大鼠给予100 mg/kg/天，均持续20天。在妊娠第20天，每组中一半的怀孕大鼠通过剖宫产取出。测量每个胎儿的形态发育参数，并对胎儿进行组织病理学、生化和基因分析。每组中其余怀孕大鼠经阴道分娩胎儿，当幼崽8周大时进行生化、组织病理学、基因和认知功能测试。给予AA对胎儿数量、胎儿体重、顶臀长度、胎盘和脑重量产生不利影响。AA对胎儿的丙二醛、还原型谷胱甘肽、总氧化剂和抗氧化状态、脑源性神经营养因子（BDNF）水平、脑组织形态、组织病理学误差评分和基因表达（BDNF/β-肌动蛋白mRNA比值）产生负面影响。给予AA导致成年大鼠的生化、组织病理学和认知功能紊乱。维生素E对胎儿和成年大鼠的神经毒性均有保护作用。我们得出结论，孕期应避免接触AA，并应摄入适量的抗氧化剂，如维生素E。
