Faculty of Medicine, Department of Medical Biochemistry, Inonu University, Malatya, Turkey.
Faculty of Medicine, Department of Histology and Embryology, Inonu University, Malatya, Turkey.
Andrologia. 2019 Aug;51(7):e13292. doi: 10.1111/and.13292. Epub 2019 Apr 17.
Thirty rats, with confirmed pregnancies by vaginal smear, were divided into five groups, each including six rats, as the Control, Corn Oil, Vitamin E, Acrylamide, Vitamin E + Acrylamide groups. The births were monitored on the 21st day to select the male rats, and the selected male rats were decapitated at the end of the 8th week. Oxidant-antioxidant parameters, serum hormone levels and histopathological examinations were performed on testis tissues of the rats. It was found that acrylamide (AA) negatively affected the serum hormone levels (Total Testosterone, Progesterone, FSH, LH, Estradiol), oxidant-antioxidant parameters in the tissues (MDA, GSH, NO, SOD, CAT, TAS, TOS) (p < 0.05) and the histological findings (the Johnson's score, seminiferous tubule diameter, histopathological images), and Vitamin E administration resulted with an increase in the total testosterone, progesterone, FSH, LH, GSH, TAS, NO, SOD, CAT levels (p < 0.05) and an improvement in histopathological findings. Currently, it is almost inevitable to be exposed to food-induced AA toxicity and such toxicity is likely to cause lifelong damage. It was concluded that Vitamin E was able to present a protective effect in the testis tissue against AA toxicity; however, further studies are necessary.
三十只怀孕大鼠通过阴道涂片法确认妊娠，将其分为五组，每组包括六只大鼠，分别为对照组、玉米油组、维生素 E 组、丙烯酰胺组、维生素 E+丙烯酰胺组。在第 21 天监测分娩，选择雄性大鼠，在第 8 周末选择的雄性大鼠断头处死。对大鼠睾丸组织进行氧化应激-抗氧化参数、血清激素水平和组织学检查。结果发现，丙烯酰胺（AA）对血清激素水平（总睾酮、孕酮、促卵泡激素、黄体生成素、雌二醇）、组织中的氧化应激-抗氧化参数（MDA、GSH、NO、SOD、CAT、TAS、TOS）（p<0.05）和组织学发现（约翰逊评分、精小管直径、组织病理学图像）产生负面影响，而维生素 E 给药导致总睾酮、孕酮、促卵泡激素、黄体生成素、GSH、TAS、NO、SOD、CAT 水平升高（p<0.05）和组织学发现改善。目前，人们几乎不可避免地会接触到食物引起的 AA 毒性，这种毒性可能会造成终生损害。结论是，维生素 E 能够对睾丸组织中的 AA 毒性产生保护作用；然而，还需要进一步的研究。
