Sun Y, Li Y, Oberley L W
Department of Biochemistry, Zhejiang Medical University, Hangchou, The People's Republic of China.
Free Radic Res Commun. 1988;4(5):299-309. doi: 10.3109/10715768809066895.
Copper, zinc superoxide dismutase (Cu,ZnSOD) and manganese superoxide dismutase (MnSOD) activities were measured in mouse large intestinal mucosa during dimethylhydrazine (DMH) carcinogenesis. Mice were divided into five groups. Group A was subcutaneously injected with DMH (20 mg/kg) weekly and fed with a diet containing 0.2% cholic acid (C) and 0.8% indole (I). Group B was injected with DMH and given indole feeding. Group C was treated with DMH injection and cholic acid feeding. Group D was given DMH injection alone. Group E was an age-matched control group given 0.9% NaCl injection. The experiment last 21 weeks. The Cu, ZnSOD activity of intestinal mucosa in group A animals began to increase significantly at the 7th week of the experiment. In groups B, C and D, however, this enzyme was not elevated statistically until the 16th week, and then each of these groups kept an increased Cu,ZnSOD level the rest of the experimental period. MnSOD activity was elevated statistically in group C animals at the 7th week. The enzyme activity in group A and D animals increased at the 9th week, but the enzyme activity did not increase statistically until the 11th week in group B. After the 16th week of the experiment the increased activity of MnSOD in all experimental groups returned to the level of the control group. Large intestinal cancer tissues had increased Cu,ZnSOD activity and decreased MnSOD activity.
在二甲基肼（DMH）致癌过程中，对小鼠大肠黏膜中的铜锌超氧化物歧化酶（Cu,ZnSOD）和锰超氧化物歧化酶（MnSOD）活性进行了测定。将小鼠分为五组。A组每周皮下注射DMH（20毫克/千克），并喂食含0.2%胆酸（C）和0.8%吲哚（I）的饮食。B组注射DMH并给予吲哚喂食。C组进行DMH注射并给予胆酸喂食。D组仅给予DMH注射。E组为年龄匹配的对照组，给予0.9%氯化钠注射。实验持续21周。A组动物肠黏膜的Cu,ZnSOD活性在实验第7周开始显著增加。然而，在B、C和D组中，该酶直到第16周才出现统计学上的升高，然后在实验剩余期间，这些组的Cu,ZnSOD水平均持续升高。C组动物的MnSOD活性在第7周出现统计学上的升高。A组和D组动物的酶活性在第9周增加，但B组直到第11周酶活性才出现统计学上的增加。实验第16周后，所有实验组中升高的MnSOD活性均恢复到对照组水平。大肠癌组织的Cu,ZnSOD活性增加，MnSOD活性降低。
