Inagake M, Yamane T, Kitao Y, Oya K, Matsumoto H, Kikuoka N, Nakatani H, Takahashi T, Nishimura H, Iwashima A
First Department of Surgery, Kyoto Prefectural University of Medicine.
Jpn J Cancer Res. 1995 Nov;86(11):1106-11. doi: 10.1111/j.1349-7006.1995.tb03027.x.
Following subcutaneous injection of 1,2-dimethylhydrazine (DMH), which is carcinogenic to rat colon and liver, to Sprague-Dawley rats, a significant increase of 8-hydroxydeoxyguanosine (8-OHdG) was observed in the DNA of colonic mucosa and liver. The 8-OHdG formation reached the maximal level at about 24 h after the DMII injection. On the other hand, no increase of 8-OHdG was observed in the DNA of the kidney. Drinking green tea extract (GTE) for ten days prior to the DMH injection significantly inhibited the formation of 8-OHdG in the colon. These findings demonstrate that DMH causes oxidative damage to the DNA of its target organ, and that GTE protects colonic mucosa from this oxidative damage.
给斯普拉格-道利大鼠皮下注射对大鼠结肠和肝脏具有致癌性的1,2-二甲基肼（DMH）后，在结肠黏膜和肝脏的DNA中观察到8-羟基脱氧鸟苷（8-OHdG）显著增加。8-OHdG的形成在DMH注射后约24小时达到最高水平。另一方面，在肾脏的DNA中未观察到8-OHdG增加。在注射DMH前饮用绿茶提取物（GTE）十天可显著抑制结肠中8-OHdG的形成。这些发现表明，DMH对其靶器官的DNA造成氧化损伤，而GTE可保护结肠黏膜免受这种氧化损伤。
