Department of Bioprocess and Polymer Engineering, School of Chemical and Energy Engineering, Faculty of Engineering, Universiti Teknologi Malaysia, Skudai, Johor, 81310, Malaysia.
Department of Chemical Engineering and Energy Sustainability, Faculty of Engineering, Universiti Malaysia Sarawak, Malaysia.
J Biotechnol. 2020 Jun 20;317:16-26. doi: 10.1016/j.jbiotec.2020.04.011. Epub 2020 Apr 27.
Bacterial pigments are potential substitute of chemical photosensitizer for dye-sensitized solar cell (DSSC) due to its non-toxic property and cost-effective production from microbial fermentation. Serratia nematodiphila YO1 was isolated from waterfall in Malaysia and identified using 16S ribosomal RNA. Characterization of the red pigment produced by the bacteria has confirmed the pigment as prodigiosin. Prodigiosin was produced from the fermentation of the bacteria in the presence of different oil substrates. Palm oil exhibited the best performance of cell growth and equivalent prodigiosin yield compared to olive oil and peanut oil. Prodigiosin produced with palm oil supplementation was 93 mg/l compared to 7.8 mg/l produced without supplementation, which recorded 11.9 times improvement. Specific growth rate of the cells improved 1.4 times when palm oil was supplemented in the medium. The prodigiosin pigment produced showed comparable performance as a DSSC sensitizer by displaying an open circuit voltage of 336.1 mV and a maximum short circuit current of 0.098 mV/cm. This study stands a novelty in proving that the production of prodigiosin is favorable in the presence of palm oil substrate with high saturated fat content, which has not been studied before. This is also among the first bacterial prodigiosin tested as photosensitizer for DSSC application.
细菌色素由于其无毒特性和从微生物发酵中经济高效地生产，是化学敏化剂的潜在替代品，可用于染料敏化太阳能电池 (DSSC)。从马来西亚的瀑布中分离到的食线虫沙雷氏菌 YO1，并使用 16S 核糖体 RNA 进行鉴定。对细菌产生的红色色素的特性进行了分析，证实该色素为灵菌红素。在存在不同油底物的情况下，通过细菌发酵生产灵菌红素。与橄榄油和花生油相比，棕榈油在细胞生长和等量灵菌红素产量方面表现出最佳性能。与没有添加棕榈油的发酵相比，添加棕榈油可将灵菌红素的产量提高 11.9 倍，达到 93mg/L。当在培养基中添加棕榈油时，细胞的比生长速率提高了 1.4 倍。所产生的灵菌红素色素作为 DSSC 敏化剂表现出相当的性能，开路电压为 336.1mV，最大短路电流为 0.098mV/cm。这项研究的新颖之处在于证明了在高饱和脂肪含量的棕榈油基质存在下，灵菌红素的生产是有利的，这在以前的研究中尚未涉及。这也是首次测试细菌灵菌红素作为 DSSC 应用的光敏剂之一。
