Liu Qianyuan, Chen Jingjing, Qin Yang, Jiang Bo, Zhang Tao
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China; International Joint Laboratory on Food Safety, Jiangnan University, Wuxi, Jiangsu 214122, China.
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China; International Joint Laboratory on Food Safety, Jiangnan University, Wuxi, Jiangsu 214122, China.
Int J Biol Macromol. 2020 Apr 27;158:461-470. doi: 10.1016/j.ijbiomac.2020.04.128.
The objective of this work is to fabricate zein/fucoidan composite nanoparticles for the delivery of pterostilbene, a hydrophobic nutraceutical with diverse beneficial biological activities. Pterostilbene-encapsulated zein/fucoidan composite nanoparticles were prepared using an anti-solvent precipitation method. The fucoidan levels affected the physicochemical properties of the composite nanoparticles. When the zein to fucoidan mass ratio was 10:1, 5:1, 2:1, or 1:1, the prepared zein/fucoidan nanoparticles were stable, and these nanoparticles showed higher pterostilbene encapsulation efficiency than did zein nanoparticles. Fucoidan-stabilized zein nanoparticles exhibited globular structure with average diameters of 120-150 nm. Fourier-transform infrared spectroscopy, X-ray diffraction, and fluorescence spectrum analysis confirmed that the formation of composite nanoparticles was mainly driven by electrostatic, hydrogen-bonding, and hydrophobic interactions between pterostilbene, zein, and fucoidan. Furthermore, the photochemical stability of pterostilbene encapsulated in zein/fucoidan nanoparticles was markedly better than that of pterostilbene loaded in zein nanoparticles or unencapsulated pterostilbene. Zein/fucoidan nanoparticles provided a better controlled release of pterostilbene than did zein nanoparticles under simulated gastrointestinal conditions. Moreover, the cytotoxicity assay demonstrated that zein/fucoidan nanoparticles were nontoxic to Caco-2, HK-2, and L-02 cells. Based on our results, the zein/fucoidan nanoparticles may be a promising delivery carrier for the encapsulation, protection, and release of pterostilbene.
本研究的目的是制备玉米醇溶蛋白/岩藻依聚糖复合纳米颗粒，用于递送芪三酚，芪三酚是一种具有多种有益生物活性的疏水性营养保健品。采用反溶剂沉淀法制备了包载芪三酚的玉米醇溶蛋白/岩藻依聚糖复合纳米颗粒。岩藻依聚糖的含量影响复合纳米颗粒的物理化学性质。当玉米醇溶蛋白与岩藻依聚糖的质量比为10:1、5:1、2:1或1:1时，制备的玉米醇溶蛋白/岩藻依聚糖纳米颗粒具有稳定性，且这些纳米颗粒比玉米醇溶蛋白纳米颗粒表现出更高的芪三酚包封效率。岩藻依聚糖稳定的玉米醇溶蛋白纳米颗粒呈现球状结构，平均直径为120 - 150 nm。傅里叶变换红外光谱、X射线衍射和荧光光谱分析证实，复合纳米颗粒的形成主要是由芪三酚、玉米醇溶蛋白和岩藻依聚糖之间的静电、氢键和疏水相互作用驱动的。此外，包封在玉米醇溶蛋白/岩藻依聚糖纳米颗粒中的芪三酚的光化学稳定性明显优于负载在玉米醇溶蛋白纳米颗粒中的芪三酚或未包封的芪三酚。在模拟胃肠道条件下，玉米醇溶蛋白/岩藻依聚糖纳米颗粒比玉米醇溶蛋白纳米颗粒能更好地控制芪三酚的释放。此外，细胞毒性试验表明，玉米醇溶蛋白/岩藻依聚糖纳米颗粒对Caco - 2、HK - 2和L - 02细胞无毒。基于我们的研究结果，玉米醇溶蛋白/岩藻依聚糖纳米颗粒可能是一种有前景的递送载体，用于芪三酚的包封、保护和释放。
