Taylor P W, Parton R
J Med Microbiol. 1977 May;10(2):225-32. doi: 10.1099/00222615-10-2-225.
Immunogel-diffusion studies showed that 60 degree C LiCl extracts of the smooth serum-resistant mutant Escherichia coli strain 17 contained greater amounts of a protein antigen than did extracts of the parent strain LP729. An extract of strain 17 was fractionated on Sepharose 4B and the protein antigen was found as the only detectable antigen in a number of fractions; sodium dodecyl sulphate-polyacrylamide gel electrophoresis indicated that these fractions contained one major polypeptide band with a molecular weight of 46 000 daltons. We suggest that this protein antigen may be partly responsible for the serum resistance of strain 17 though its presence in other serum-sensitive strains suggests that additional factors are essential for full serum resistance.
免疫凝胶扩散研究表明，光滑血清抗性突变型大肠杆菌菌株17在60摄氏度的氯化锂提取物中所含的一种蛋白质抗原量比亲本菌株LP729的提取物更多。菌株17的提取物在琼脂糖4B上进行分级分离，发现该蛋白质抗原是多个级分中唯一可检测到的抗原；十二烷基硫酸钠-聚丙烯酰胺凝胶电泳表明，这些级分含有一条分子量为46000道尔顿的主要多肽带。我们认为，这种蛋白质抗原可能部分导致了菌株17的血清抗性，尽管它也存在于其他血清敏感菌株中，这表明还需要其他因素才能实现完全的血清抗性。
