Department of Bromatology, Faculty of Pharmacy, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, Poland.
Nutrients. 2020 Apr 27;12(5):1232. doi: 10.3390/nu12051232.
Fatty acids, especially polyunsaturated, and their metabolites (eicosanoids) play many pivotal roles in human body, influencing various physiological and pathological processes. The aim of the study was to evaluate the effect of supplementation with edible oils diverse in terms of fatty acid composition on fatty acid contents, activities of converting their enzymes, and on lipoxygenase metabolites of arachidonic and linoleic acids (eicosanoids) in rat serum. Female Sprague-Dawley rats divided into seven groups were used in the study. Animals from six groups were fed one of oils daily (carotino oil, made up by combining of red palm oil and canola oil, linseed oil, olive oil, rice oil, sesame oil, or sunflower oil). One group received a standard diet only. Fatty acids were determined using gas chromatography with flame ionization detection. Eicosanoids-hydroxyeicosatetraenoic (HETE) and hydroxyoctadecadienoic acids (HODE) were extracted using a solid-phase extraction method and analyzed with HPLC. Vegetable oils given daily to rats caused significant changes in serum fatty acid profile and eicosanoid concentrations. Significant differences were also found in desaturases' activity, with the linseed and olive oil supplemented groups characterized by the highest D6D and D5D activity. These findings may play a significant role in various pathological states.
脂肪酸，特别是多不饱和脂肪酸及其代谢物（类二十烷酸）在人体中发挥着许多关键作用，影响着各种生理和病理过程。本研究旨在评估不同脂肪酸组成的食用油补充对大鼠血清中脂肪酸含量、其转化酶活性以及花生四烯酸和亚油酸（类二十烷酸）的脂氧合酶代谢物的影响。本研究使用了 7 组雌性 Sprague-Dawley 大鼠。其中 6 组动物每天喂食一种油（胡萝卜油，由红棕榈油和菜籽油组合而成，亚麻籽油，橄榄油，米糠油，芝麻油或葵花籽油）。一组只接受标准饮食。使用火焰离子化检测气相色谱法测定脂肪酸。通过固相萃取法提取类二十烷酸-羟二十碳四烯酸（HETE）和羟十八碳二烯酸（HODE），并用 HPLC 进行分析。每日给予大鼠的植物油导致血清脂肪酸谱和类二十烷酸浓度发生显著变化。去饱和酶活性也存在显著差异，补充亚麻籽油和橄榄油的组表现出最高的 D6D 和 D5D 活性。这些发现可能在各种病理状态中发挥重要作用。
