Jelińska Małgorzata, Białek Agnieszka, Mojska Hanna, Gielecińska Iwona, Tokarz Andrzej
Department of Bromatology, Faculty of Pharmacy with the Laboratory Medicine Division, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, Poland.
Department of Food and Food Supplements, National Food and Nutrition Institute, Powsińska 61/63, 02-903 Warsaw, Poland.
Biochim Biophys Acta. 2014 Nov;1842(11):2230-6. doi: 10.1016/j.bbadis.2014.08.013. Epub 2014 Sep 6.
Conjugated linoleic acid (CLA) is thought to exert anticarcinogenic, antiatherogenic, anti-inflammatory and weight loss effects. The impact on eicosanoid biosynthesis may be one of the mechanisms of its action. The aim of this study was to establish whether CLA mixture supplemented daily after administration of carcinogen (7, 12-dimethylbenz[a]anthracene, DMBA) influenced the concentration of linoleic and arachidonic acid metabolites: 13- or 9-hydroxyoctadecadienoic acids (13-, 9-HODE) and 15-, 12- or 5-hydroxyeicosatetraenoic acids (15-, 12- or 5-HETE) and prostaglandin E2 (PGE2) in rat serum and DMBA-induced tumours. The correlations between polyunsaturated fatty acids (PUFA) and HETE and HODE contents in serum were also investigated. Female Sprague-Dawley rats divided into three groups according to the diet (1% Bio-C.L.A., 2% Bio-C.L.A. and plant oil in the control group) were used in the study. On the 50th day of life some of the animals in every dietary group were administered DMBA to induce tumours. Since that day, the rats were fed one of the above-mentioned diets. After 15weeks the animals were sacrificed and blood and tumours were collected. HETE and HODE were extracted using a solid-phase extraction (SPE) method on C18 columns and analysed with LC-MS/MS. The results of our study showed that CLA daily supplementation after carcinogen administration influence LA and AA metabolite levels in serum and tumours. However, the ratios of eicosanoids having opposite effects (e.g. 12-HETE/15-HETE), not concentrations of particular compounds, appear to be better indicators of pathological processes.
共轭亚油酸（CLA）被认为具有抗癌、抗动脉粥样硬化、抗炎和减肥作用。对类二十烷酸生物合成的影响可能是其作用机制之一。本研究的目的是确定在给予致癌物（7,12-二甲基苯并[a]蒽，DMBA）后每日补充CLA混合物是否会影响大鼠血清和DMBA诱导的肿瘤中亚油酸和花生四烯酸代谢物的浓度：13-或9-羟基十八碳二烯酸（13-，9-HODE）以及15-、12-或5-羟基二十碳四烯酸（15-、12-或5-HETE）和前列腺素E2（PGE2）。还研究了血清中多不饱和脂肪酸（PUFA）与HETE和HODE含量之间的相关性。本研究使用了根据饮食分为三组的雌性Sprague-Dawley大鼠（对照组为植物油，1%生物CLA组，2%生物CLA组）。在出生后第50天，每个饮食组的一些动物被给予DMBA以诱导肿瘤。从那天起，大鼠喂食上述饮食之一。15周后处死动物并收集血液和肿瘤。使用C18柱上的固相萃取（SPE）方法提取HETE和HODE，并用LC-MS/MS进行分析。我们的研究结果表明，致癌物给药后每日补充CLA会影响血清和肿瘤中LA和AA代谢物水平。然而，具有相反作用的类二十烷酸的比例（例如12-HETE/15-HETE），而非特定化合物的浓度，似乎是病理过程的更好指标。
