Oshio S
Department of Biology, College of Arts and Sciences, University of Tokyo, Japan.
Gamete Res. 1988 Jun;20(2):159-64. doi: 10.1002/mrd.1120200206.
The apparent densities of sperm cells in several mammalian species were determined by means of Percoll density-gradient centrifugation. Semen samples were obtained from (1) ejaculates from the bull, human, and rabbit, and (2) from the cauda epididymis of the goat, golden hamster, house musk shrew, mastomys, and mouse. The profiles of sperm distribution showed a single peak after the centrifugation in the first group and goat, whereas two separate main peaks in the second group (except goat). This disparity in sperm distribution profiles may be due mainly to differences in the degree of maturity of the sperm. Highly motile and mature sperm were obtained at higher densities, whereas immotile or immature sperm were found at the apparent densities. Thus, in mammals, the profiles of sperm distribution in the Percoll density gradient are classified into two types, those with a single peak and those with two separate peaks. The apparent density of sperm cells may be of importance in sperm physiology.
通过Percoll密度梯度离心法测定了几种哺乳动物精子细胞的表观密度。精液样本取自：（1）公牛、人类和兔子的射精，以及（2）山羊、金黄地鼠、家麝鼩、非洲沼鼠和小鼠的附睾尾部。精子分布曲线显示，第一组和山羊离心后出现单峰，而第二组（山羊除外）出现两个独立的主峰。精子分布曲线的这种差异可能主要归因于精子成熟程度的不同。高活力和成熟的精子在较高密度处获得，而无活力或未成熟的精子则在表观密度处发现。因此，在哺乳动物中，Percoll密度梯度中的精子分布曲线分为两种类型，即单峰型和双峰型。精子细胞的表观密度在精子生理学中可能具有重要意义。
