Aix Marseille Univ., IRD, AP-HM, SSA, VITROME, 13005 Marseille, France - IHU-Méditerranée Infection, 19-21 Boulevard Jean Moulin, 13005 Marseille, France.
Université Chadli Bendjdid, Département des sciences Vétérinaire, 36000 El Tarf, Algeria.
Parasite. 2020;27:28. doi: 10.1051/parasite/2020026. Epub 2020 Apr 30.
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is now routinely used for the rapid identification of microorganisms isolated from clinical samples and has been recently successfully applied to the identification of arthropods. In the present study, this proteomics tool was used to identify lice collected from livestock and poultry in Algeria. The MALDI-TOF MS spectra of 408 adult specimens were measured for 14 species, including Bovicola bovis, B. ovis, B. caprae, Haematopinus eurysternus, Linognathus africanus, L. vituli, Solenopotes capillatus, Menacanthus stramineus, Menopon gallinae, Chelopistes meleagridis, Goniocotes gallinae, Goniodes gigas, Lipeurus caponis and laboratory reared Pediculus humanus corporis. Good quality spectra were obtained for 305 samples. Spectral analysis revealed intra-species reproducibility and inter-species specificity that were consistent with the morphological classification. A blind test of 248 specimens was performed against the in-lab database upgraded with new spectra and validated using molecular tools. With identification percentages ranging from 76% to 100% alongside high identification scores (mean = 2.115), this study proposes MALDI-TOF MS as an effective tool for discriminating lice species.
基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）现已广泛用于快速鉴定从临床样本中分离出的微生物，并已成功应用于节肢动物的鉴定。在本研究中，该蛋白质组学工具用于鉴定从阿尔及利亚家畜和家禽身上采集的虱子。对包括牛皮虱、绵羊虱、山羊虱、马毛虱、非洲毛虱、牛虱、细角毛虱、粗角毛虱、鸡皮刺螨、鸡羽虱、禽刺螨、鸡皮蝇、巨皮蝇、鸡巨螯螨和实验室饲养的人体虱在内的 14 个物种的 408 个成虫标本进行了 MALDI-TOF MS 光谱测量。对 305 个样本获得了高质量的光谱。光谱分析显示出种内重现性和种间特异性，与形态分类一致。对 248 个样本进行了盲测，该数据库是在升级了新的光谱并使用分子工具验证后建立的。该研究采用 MALDI-TOF MS 对虱子物种进行了鉴定，其鉴定百分比在 76%至 100%之间，鉴定得分高（平均值为 2.115），证明 MALDI-TOF MS 是一种有效的虱子物种鉴别工具。
