• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

转录因子 T-bet 在小鼠和人类中区分具有不同组织分布和抗体特异性的记忆 B 细胞亚群。

The Transcription Factor T-bet Resolves Memory B Cell Subsets with Distinct Tissue Distributions and Antibody Specificities in Mice and Humans.

机构信息

Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.

La Jolla Institute for Allergy and Immunology, La Jolla, CA 92037, USA.

出版信息

Immunity. 2020 May 19;52(5):842-855.e6. doi: 10.1016/j.immuni.2020.03.020. Epub 2020 Apr 29.

DOI:10.1016/j.immuni.2020.03.020
PMID:32353250
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7242168/
Abstract

B cell subsets expressing the transcription factor T-bet are associated with humoral immune responses and autoimmunity. Here, we examined the anatomic distribution, clonal relationships, and functional properties of T-bet and T-bet memory B cells (MBCs) in the context of the influenza-specific immune response. In mice, both T-bet and T-bet hemagglutinin (HA)-specific B cells arose in germinal centers, acquired memory B cell markers, and persisted indefinitely. Lineage tracing and IgH repertoire analyses revealed minimal interconversion between T-bet and T-bet MBCs, and parabionts showed differential tissue residency and recirculation properties. T-bet MBCs could be subdivided into recirculating T-bet MBCs and spleen-resident T-bet MBCs. Human MBCs displayed similar features. Conditional gene deletion studies revealed that T-bet expression in B cells was required for nearly all HA stalk-specific IgG2c antibodies and for durable neutralizing titers to influenza. Thus, T-bet expression distinguishes MBC subsets that have profoundly different homing, residency, and functional properties, and mediate distinct aspects of humoral immune memory.

摘要

B 细胞亚群表达转录因子 T-bet 与体液免疫反应和自身免疫有关。在这里，我们研究了流感特异性免疫反应背景下 T-bet 和 T-bet 记忆 B 细胞（MBC）的解剖分布、克隆关系和功能特性。在小鼠中，T-bet 和 T-bet 血凝素（HA）特异性 B 细胞均在生发中心中出现，获得了记忆 B 细胞标志物，并无限期持续存在。谱系追踪和 IgH 库分析显示 T-bet 和 T-bet MBC 之间几乎没有相互转换，而联体动物显示出不同的组织驻留和再循环特性。T-bet MBC 可进一步分为循环 T-bet MBC 和脾脏驻留 T-bet MBC。人类 MBC 表现出类似的特征。条件基因缺失研究表明，B 细胞中 T-bet 的表达几乎是所有 HA 茎特异性 IgG2c 抗体和流感病毒持久中和滴度所必需的。因此，T-bet 的表达区分了具有截然不同归巢、驻留和功能特性的 MBC 亚群，并介导了体液免疫记忆的不同方面。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/65e8/7242168/5d8b89789b84/nihms-1584982-f0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/65e8/7242168/38c100125cf6/nihms-1584982-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/65e8/7242168/da684079cb03/nihms-1584982-f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/65e8/7242168/636957abe7b4/nihms-1584982-f0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/65e8/7242168/544521832015/nihms-1584982-f0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/65e8/7242168/5d8b89789b84/nihms-1584982-f0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/65e8/7242168/38c100125cf6/nihms-1584982-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/65e8/7242168/da684079cb03/nihms-1584982-f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/65e8/7242168/636957abe7b4/nihms-1584982-f0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/65e8/7242168/544521832015/nihms-1584982-f0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/65e8/7242168/5d8b89789b84/nihms-1584982-f0006.jpg

相似文献

1
The Transcription Factor T-bet Resolves Memory B Cell Subsets with Distinct Tissue Distributions and Antibody Specificities in Mice and Humans.转录因子 T-bet 在小鼠和人类中区分具有不同组织分布和抗体特异性的记忆 B 细胞亚群。
Immunity. 2020 May 19;52(5):842-855.e6. doi: 10.1016/j.immuni.2020.03.020. Epub 2020 Apr 29.
2
T-bet Memory B Cells Stay in Place.T 细胞记忆 B 细胞原地停留。
Immunity. 2020 May 19;52(5):726-728. doi: 10.1016/j.immuni.2020.04.013.
3
Overexpression of T-bet in HIV infection is associated with accumulation of B cells outside germinal centers and poor affinity maturation.HIV 感染中 T-bet 的过度表达与生发中心外 B 细胞的积累和亲和力成熟不良有关。
Sci Transl Med. 2019 Nov 27;11(520). doi: 10.1126/scitranslmed.aax0904.
4
Assembly of a spatial circuit of T-bet-expressing T and B lymphocytes is required for antiviral humoral immunity.表达T-bet的T淋巴细胞和B淋巴细胞的空间回路组装是抗病毒体液免疫所必需的。
Sci Immunol. 2021 Jun 11;6(60). doi: 10.1126/sciimmunol.abi4710.
5
T-bet memory B cells: Generation, function, and fate.T 细胞脑啡肽酶记忆 B 细胞：生成、功能和命运。
Immunol Rev. 2019 Mar;288(1):149-160. doi: 10.1111/imr.12736.
6
Divergent transcriptional programming of class-specific B cell memory by T-bet and RORα.T-bet 和 RORα 对特异性 B 细胞记忆的分化转录编程。
Nat Immunol. 2012 May 6;13(6):604-11. doi: 10.1038/ni.2294.
7
Role of CD11c T-bet B cells in human health and disease.CD11c T-bet B细胞在人类健康与疾病中的作用。
Cell Immunol. 2017 Nov;321:40-45. doi: 10.1016/j.cellimm.2017.05.008. Epub 2017 Jul 11.
8
Ex vivo analysis of human memory B lymphocytes specific for A and B influenza hemagglutinin by polychromatic flow-cytometry.通过多色流式细胞术分析针对 A 和 B 型流感血凝素的人记忆 B 淋巴细胞的体外分析。
PLoS One. 2013 Aug 15;8(8):e70620. doi: 10.1371/journal.pone.0070620. eCollection 2013.
9
T-bet-expressing B cells during HIV and HCV infections.HIV和HCV感染期间表达T-bet的B细胞。
Cell Immunol. 2017 Nov;321:26-34. doi: 10.1016/j.cellimm.2017.04.012. Epub 2017 Jul 11.
10
The Differentiation of Human Tonsil B Cells With the Phenotypic and Functional Characteristics of T-bet+ Atypical Memory B Cells in Malaria.疟疾中具有 T-bet+ 非典型记忆 B 细胞表型和功能特征的人扁桃体 B 细胞的分化。
Front Immunol. 2019 Apr 24;10:852. doi: 10.3389/fimmu.2019.00852. eCollection 2019.

引用本文的文献

1
Multimodal profiling reveals tissue-directed signatures of human immune cells altered with age.多模态分析揭示了随年龄变化的人类免疫细胞的组织定向特征。
Nat Immunol. 2025 Aug 13. doi: 10.1038/s41590-025-02241-4.
2
TNF inhibitors affect the induction and maintenance of spike-specific B-cell responses after mRNA vaccination.肿瘤坏死因子抑制剂会影响mRNA疫苗接种后刺突特异性B细胞反应的诱导和维持。
RMD Open. 2025 Aug 4;11(3):e005724. doi: 10.1136/rmdopen-2025-005724.
3
Elucidating the characteristics and clonal evolutionary trajectory of influenza neuraminidase broadly reactive B cell.

本文引用的文献

1
Overexpression of T-bet in HIV infection is associated with accumulation of B cells outside germinal centers and poor affinity maturation.HIV 感染中 T-bet 的过度表达与生发中心外 B 细胞的积累和亲和力成熟不良有关。
Sci Transl Med. 2019 Nov 27;11(520). doi: 10.1126/scitranslmed.aax0904.
2
Context-Dependent Role for T-bet in T Follicular Helper Differentiation and Germinal Center Function following Viral Infection.病毒感染后 T-bet 在 T 滤泡辅助细胞分化和生发中心功能中的上下文相关作用。
Cell Rep. 2019 Aug 13;28(7):1758-1772.e4. doi: 10.1016/j.celrep.2019.07.034.
3
Activation Dynamics and Immunoglobulin Evolution of Pre-existing and Newly Generated Human Memory B cell Responses to Influenza Hemagglutinin.
阐明流感病毒神经氨酸酶广泛反应性B细胞的特征和克隆进化轨迹。
Sci Adv. 2025 Aug;11(31):eadx4810. doi: 10.1126/sciadv.adx4810. Epub 2025 Aug 1.
4
Transcription factor T-bet regulates the maintenance and differentiation potential of lymph node and lung effector memory B cell subsets.转录因子T-bet调节淋巴结和肺效应记忆B细胞亚群的维持和分化潜能。
Immunity. 2025 Jul 8;58(7):1706-1724.e6. doi: 10.1016/j.immuni.2025.05.021. Epub 2025 Jun 21.
5
Early multiple sclerosis activity associated with TBX21+CD21loCXCR3+ B cell expansion resembling EBV-induced phenotypes.早期多发性硬化活动与TBX21+CD21loCXCR3+ B细胞扩增相关，类似于EB病毒诱导的表型。
JCI Insight. 2025 May 13;10(12). doi: 10.1172/jci.insight.188543. eCollection 2025 Jun 23.
6
Critical roles of chronic BCR signaling in the differentiation of anergic B cells into age-associated B cells in aging and autoimmunity.慢性B细胞受体信号在衰老和自身免疫过程中无反应性B细胞分化为年龄相关B细胞中的关键作用。
Sci Adv. 2025 Apr 18;11(16):eadt8199. doi: 10.1126/sciadv.adt8199.
7
Coordinated Regulation of Extrafollicular B Cell Responses by IL-12 and IFNγ.IL-12和IFNγ对滤泡外B细胞反应的协同调节
Immunol Rev. 2025 May;331(1):e70027. doi: 10.1111/imr.70027.
8
EZH2 coordinates memory B-cell programming and recall responses.EZH2协调记忆B细胞编程和回忆反应。
J Immunol. 2025 May 1;214(5):947-957. doi: 10.1093/jimmun/vkaf004.
9
T-bet-expressing B cells promote atherosclerosis in apolipoprotein E-deficient mice.表达T-bet的B细胞促进载脂蛋白E缺陷小鼠的动脉粥样硬化。
J Immunol. 2025 Feb 26;214(3):317-24. doi: 10.1093/jimmun/vkae027.
10
Activation of CXCR3 Tfh cells and B cells in lymph nodes during acute HIV-1 infection correlates with HIV-specific antibody development.急性HIV-1感染期间淋巴结中CXCR3辅助性滤泡T细胞和B细胞的激活与HIV特异性抗体的产生相关。
J Virol. 2025 Mar 18;99(3):e0153224. doi: 10.1128/jvi.01532-24. Epub 2025 Feb 11.
预先存在和新产生的人类记忆 B 细胞对流感血凝素反应的激活动态和免疫球蛋白进化。
Immunity. 2019 Aug 20;51(2):398-410.e5. doi: 10.1016/j.immuni.2019.06.024. Epub 2019 Jul 23.
4
A Facile Method for the Removal of dsRNA Contaminant from In Vitro-Transcribed mRNA.一种从体外转录的mRNA中去除双链RNA污染物的简便方法。
Mol Ther Nucleic Acids. 2019 Apr 15;15:26-35. doi: 10.1016/j.omtn.2019.02.018. Epub 2019 Feb 27.
5
The establishment of resident memory B cells in the lung requires local antigen encounter.肺中定居记忆 B 细胞的建立需要局部抗原的接触。
Nat Immunol. 2019 Jan;20(1):97-108. doi: 10.1038/s41590-018-0260-6. Epub 2018 Dec 3.
6
Distinct Effector B Cells Induced by Unregulated Toll-like Receptor 7 Contribute to Pathogenic Responses in Systemic Lupus Erythematosus.未调节的 Toll 样受体 7 诱导的效应 B 细胞导致系统性红斑狼疮的致病性反应。
Immunity. 2018 Oct 16;49(4):725-739.e6. doi: 10.1016/j.immuni.2018.08.015. Epub 2018 Oct 9.
7
ImmuneDB, a Novel Tool for the Analysis, Storage, and Dissemination of Immune Repertoire Sequencing Data.免疫数据库（ImmuneDB）：一种用于免疫受体测序数据分析、存储和传播的新型工具。
Front Immunol. 2018 Sep 21;9:2107. doi: 10.3389/fimmu.2018.02107. eCollection 2018.
8
Nucleoside-modified mRNA immunization elicits influenza virus hemagglutinin stalk-specific antibodies.核苷修饰的 mRNA 免疫可诱导流感病毒血凝素茎特异性抗体。
Nat Commun. 2018 Aug 22;9(1):3361. doi: 10.1038/s41467-018-05482-0.
9
T-Bet IgM Memory Cells Generate Multi-lineage Effector B Cells.T 细胞特异性转录因子 IgM 记忆细胞产生多谱系效应 B 细胞。
Cell Rep. 2018 Jul 24;24(4):824-837.e3. doi: 10.1016/j.celrep.2018.06.074.
10
Computational Evaluation of B-Cell Clone Sizes in Bulk Populations.批量群体中B细胞克隆大小的计算评估
Front Immunol. 2018 Jun 29;9:1472. doi: 10.3389/fimmu.2018.01472. eCollection 2018.