Shandong Provincial Key Laboratoryof Synthetic Biology, Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China.
Shandong Provincial Key Laboratoryof Synthetic Biology, Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China; University of Chinese Academy of Sciences, Beijing 100039, China; Egyptian Petroleum Research Institute, Nasr City 11727, Cairo, Egypt.
Environ Res. 2020 Jul;186:109563. doi: 10.1016/j.envres.2020.109563. Epub 2020 Apr 22.
The purpose of this study was to improve methane generation from corn stalk (CS) through alkaline hydrogen peroxide and lithium chloride/N,N-dimethylacetamide (AHP-LiCl/DMAc) pretreatment. Changes in the structures of treated and untreated CSs were investigated, and biomass components, including cellulose, hemicellulose and lignin, were analysed. Our findings revealed that AHP-LiCl/DMAc pretreatment improved accumulative methane yield by forceful delignification and effectively destroyed the structure of cellulose. The AHP-LiCl/DMAc-treated group had a maximum methane yield of 318.6 ± 17.85 mL/g volatile solid, which was 40.08% and 10.10% higher than the maximum methane yields of the untreated and AHP-treated group, respectively. This result showed enhanced cellulose dissolution by the ionic solvent of LiCl/DMAc and improved enzymatic saccharification in fermentative bacteria without structural modifications. The AHP-LiCl/DMAc treated group had higher glucose level, acetate followed by biomethanation process. Furthermore, the decrease in crystallinity indexes for AHP-LiCl/DMAc treated group was reported. Overall, this investigation proved a promising pretreatment approach for enhancing the degradation of CS into reducing sugars and improving methane generation.
本研究旨在通过碱性过氧化氢和氯化锂/二甲基乙酰胺（AHP-LiCl/DMAc）预处理来提高玉米秸秆（CS）的甲烷生成量。研究了处理前后 CS 结构的变化，并分析了生物质成分，包括纤维素、半纤维素和木质素。研究结果表明，AHP-LiCl/DMAc 预处理通过强力脱木质素提高了累积甲烷产量，并有效地破坏了纤维素的结构。AHP-LiCl/DMAc 处理组的最大甲烷产量为 318.6±17.85 mL/g 挥发性固体，分别比未处理组和 AHP 处理组的最大甲烷产量高 40.08%和 10.10%。这一结果表明，LiCl/DMAc 的离子溶剂增强了纤维素的溶解，并在不改变结构的情况下提高了发酵细菌的酶解糖化作用。AHP-LiCl/DMAc 处理组的葡萄糖水平更高，随后是乙酸，然后是生物甲烷化过程。此外，还报道了 AHP-LiCl/DMAc 处理组结晶度指数的降低。总的来说，这项研究证明了 AHP-LiCl/DMAc 预处理是一种很有前途的方法，可以提高 CS 降解为还原糖的效率，并提高甲烷的生成量。
