J-Lat 10.6中的整合型1型人类免疫缺陷病毒序列
Integrated Human Immunodeficiency Virus Type 1 Sequence in J-Lat 10.6.
作者信息
Chung Cheng-Han, Mele Anthony R, Allen Alexander G, Costello Robert, Dampier Will, Nonnemacher Michael R, Wigdahl Brian
机构信息
Department of Microbiology and Immunology, Drexel University College of Medicine, Philadelphia, Pennsylvania, USA.
Center for Molecular Virology and Translational Neuroscience, Institute for Molecular Medicine and Infectious Disease, Drexel University College of Medicine, Philadelphia, Pennsylvania, USA.
出版信息
Microbiol Resour Announc. 2020 Apr 30;9(18):e00179-20. doi: 10.1128/MRA.00179-20.
Abstract
The full length of HIV/R7/E/GFP integrated in the J-Lat 10.6 cell line was sequenced in this study. The single copy of the integrated virus, including the breakpoints from the human chromosome to the provirus, was amplified by two separate PCRs. A 10,200-bp genome sequence was acquired, analyzed, and deposited in GenBank.
摘要
本研究对整合于J-Lat 10.6细胞系中的HIV/R7/E/GFP全长进行了测序。通过两次独立的聚合酶链式反应（PCR）扩增整合病毒的单拷贝，包括从人类染色体到前病毒的断点。获得了一个10200碱基对的基因组序列，进行了分析并提交至基因库。
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Sci Rep. 2018 May 17;8(1):7784. doi: 10.1038/s41598-018-26190-1.
2
CRISPR/Cas9 Inhibits Multiple Steps of HIV-1 Infection.CRISPR/Cas9 抑制 HIV-1 感染的多个步骤。
Hum Gene Ther. 2018 Nov;29(11):1264-1276. doi: 10.1089/hum.2018.018. Epub 2018 May 9.
3
Designing broad-spectrum anti-HIV-1 gRNAs to target patient-derived variants.设计广谱抗 HIV-1 gRNAs 以靶向患者来源的变异体。
Sci Rep. 2017 Oct 31;7(1):14413. doi: 10.1038/s41598-017-12612-z.
4
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EBioMedicine. 2017 Jul;21:170-181. doi: 10.1016/j.ebiom.2017.06.011. Epub 2017 Jun 13.
5
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