Vink C, Groenink M, Elgersma Y, Fouchier R A, Tersmette M, Plasterk R H
Division of Molecular Biology, Netherlands Cancer Institute, Amsterdam.
J Virol. 1990 Nov;64(11):5626-7. doi: 10.1128/JVI.64.11.5626-5627.1990.
Integrated retroviral DNA is flanked by short direct repeats of the target DNA. The length of these repeats is specific for the provirus that is integrated (H.E. Varmus, in J.A. Shapiro, ed., Mobile Genetic Elements, 1983). For the human immunodeficiency virus type I (HIV-1), the length of the direct repeats in the target DNA was shown to be 5 bp in one case (Muesing et al., Nature [London] 313:450-458, 1985) and 7 bp in another (Starcich et al., Science 227:538-540, 1985). One possible explanation for this discrepancy is that the direct repeats flanking HIV-1 proviruses are variable. To investigate this, we analyzed the junctions between HIV-1 proviral DNA and human DNA from nine individual clones. In each clone the provirus was flanked by a 5-bp direct repeat of human DNA. Analysis of the proviral clone previously described as being flanked by a 7-bp direct repeat of target DNA (Starcich et al., op. cit.) revealed that this clone was flanked by a 5-bp repeat instead. Therefore, we conclude that HIV-1 proviruses are flanked by 5-bp direct repeats of human DNA. The sequences of the 5-bp duplications from the different proviral clones do not have any apparent similarity to each other or to HIV-1 DNA.
整合的逆转录病毒DNA两侧是靶DNA的短直接重复序列。这些重复序列的长度对于整合的原病毒来说是特定的（H.E.瓦尔默斯，载于J.A.夏皮罗编，《移动遗传元件》，1983年）。对于人类免疫缺陷病毒1型（HIV-1），在一个案例中，靶DNA中的直接重复序列长度显示为5个碱基对（缪辛等人，《自然》[伦敦]313:450 - 458，1985年），而在另一个案例中为7个碱基对（斯塔西奇等人，《科学》227:538 - 540，1985年）。这种差异的一种可能解释是，HIV-1原病毒两侧的直接重复序列是可变的。为了研究这一点，我们分析了来自九个独立克隆的HIV-1原病毒DNA与人类DNA之间的连接。在每个克隆中，原病毒两侧都是人类DNA的5个碱基对的直接重复序列。对先前描述为两侧是靶DNA的7个碱基对直接重复序列的原病毒克隆（斯塔西奇等人，同前）的分析表明，该克隆两侧实际上是5个碱基对的重复序列。因此，我们得出结论，HIV-1原病毒两侧是人类DNA的5个碱基对的直接重复序列。来自不同原病毒克隆的5个碱基对重复序列的序列彼此之间或与HIV-1 DNA没有任何明显的相似性。
