Gazdag M, Szepesi G, Szeleczki E
Chemical Works of Gedeon Richter, Ltd., Budapest, Hungary.
J Chromatogr. 1988 Nov 11;454:83-94. doi: 10.1016/s0021-9673(00)88604-5.
The application of solvent optimization to the development of isocratic reversed-phase liquid chromatography has been reported in several publications. Two different approaches to solvent optimization for controlling band spacing for the maximum resolution of samples are "solvent strength" and "solvent type" optimization. To improve the separation selectivity further the combination of these two approaches was examined, as a (global) optimum mobile phase composition requires the optimization of the solvent strength by varying the percentage of organic component and of the solvent selectivity of methanol, acetonitrile, tetrahydrofuran and water. It was found that the combination of "solvent strength" and "solvent type" optimization provides a markedly better separation than either procedure alone.
溶剂优化在等度反相液相色谱法开发中的应用已在多篇出版物中有所报道。控制谱带间距以实现样品最大分离度的两种不同溶剂优化方法是“溶剂强度”和“溶剂类型”优化。为了进一步提高分离选择性，研究了这两种方法的组合，因为（全局）最佳流动相组成需要通过改变有机组分的百分比以及甲醇、乙腈、四氢呋喃和水的溶剂选择性来优化溶剂强度。结果发现，“溶剂强度”和“溶剂类型”优化相结合所提供的分离效果明显优于单独使用任何一种方法。
