Gazdag M, Szepesi G, Fábián-Varga K
Chemical Works of Gedeon Richter, Ltd., Budapest, Hungary.
J Chromatogr. 1988 Nov 11;454:95-107. doi: 10.1016/s0021-9673(00)88605-7.
Optimization of selectivity in normal-phase chromatography was studied. Using the same optimization criteria (Rs,min, Dmin, Rsb and Rsa) as in Part I the possible combination of "solvent strength" optimization by variation of the percentage of polar modifier in the less polar mobile phase and "solvent type" optimization by measuring the solvent selectivity of chloroform, acetonitrile, tetrahydrofuran and dioxane was examined. From the results it can be concluded that by replacement of medium-polarity solvents (chloroform, acetonitrile, tetrahydrofuran and dioxane) in the mobile phase, the selectivity of the separation can be significantly improved as the elution order is a function of solvent type belonging to Class P. In the separation of a steroid mixture dioxane provides the best properties for improving band spacing.
研究了正相色谱中选择性的优化。采用与第一部分相同的优化标准（Rs,min、Dmin、Rsb和Rsa），通过改变极性较小的流动相中极性改性剂的百分比来优化“溶剂强度”，并通过测量氯仿、乙腈、四氢呋喃和二氧六环的溶剂选择性来优化“溶剂类型”，考察了这两种优化方式的可能组合。从结果可以得出结论，通过更换流动相中的中等极性溶剂（氯仿、乙腈、四氢呋喃和二氧六环），分离的选择性可以显著提高，因为洗脱顺序是属于P类溶剂类型的函数。在甾体混合物的分离中，二氧六环在改善谱带间距方面具有最佳性能。
