Department of Pathology and Clinical Propaedeutics, School of Dentistry, São Paulo State University (UNESP), Rua José Bonifácio, 1193, Vila Mendonça, Aracatuba, Sao Paulo, 16015-050, Brazil.
Department of Basic Sciences, School of Dentistry, São Paulo State University (UNESP), Aracatuba, Sao Paulo, Brazil.
Clin Oral Investig. 2020 Dec;24(12):4283-4290. doi: 10.1007/s00784-020-03290-9. Epub 2020 Apr 30.
This study aimed to evaluate the effects of chronic consumption of cachaça, a Brazilian beverage containing alcohol, on submandibular glands (SM) of rats by using histomorphometric and biochemical parameters.
Twenty-four male rats (40 days of age) were assigned into the following four groups (n = 6): two control groups for 75 days (C75) and 105 days (C105), and two experimental groups of cachaça ingestion with ascending concentrations for consecutive 75 days (CA75) and 105 days (CA105). On the right SM glands, the striated, granular and acini ducts were processed for histomorphometric analysis. The left SM glands were weighed and stored at - 80 °C, to evaluate through biochemical tests carried out by spectrophotometric methods, the functional activity of total acid phosphatase (TAP), tartrate-resistant acid phosphatase (TRAP), and alkaline phosphatase (ALP) and to determine the mucin levels.
The absolute and relative weights of the SM glands in both experimental groups were reduced in relation to the controls (p < 0.05). The histomorphometric analysis showed a significant reduction of the acini area (p < 0.05) and non-relevant reduction of striated ducts (p > 0.05). The granular ducts did not show a significant increase of the area (p > 0.05). The TAP and TRAP activities were significantly decreased in the experimental groups (p < 0.05), while the ALP functional activity decreased moderately (p > 0.05). Mucin levels also had a significant reduction when compared with the control groups (p < 0.05).
Chronic consumption of cachaça can cause morphological changes associated with glandular atrophy, loss of biochemical functionality of phosphatases, and the reduction of mucin synthesis.
The consumption of cachaça can compromise the functions of the submandibular glands by altering their morphology and enzymatic activity.
本研究旨在通过组织形态计量学和生化参数评估巴西酒精饮料甘蔗酒（cachaça）的慢性摄入对颌下腺（SM）的影响。
将 24 只 40 天大的雄性大鼠分为以下四组（n=6）：75 天（C75）和 105 天（C105）两个对照组，以及连续摄入甘蔗酒浓度递增 75 天（CA75）和 105 天（CA105）的两个实验组。在右侧 SM 腺体上，对纹状、颗粒和腺泡导管进行组织形态计量学分析。左侧 SM 腺体称重并储存在-80°C，通过分光光度法进行生化测试，评估总酸性磷酸酶（TAP）、耐酒石酸酸性磷酸酶（TRAP）和碱性磷酸酶（ALP）的功能活性，并测定粘蛋白水平。
与对照组相比，两个实验组的 SM 腺体的绝对和相对重量均降低（p<0.05）。组织形态计量学分析显示，腺泡面积显著减小（p<0.05），纹状导管非相关减小（p>0.05）。颗粒状导管面积无明显增加（p>0.05）。实验组的 TAP 和 TRAP 活性显著降低（p<0.05），而 ALP 功能活性中度降低（p>0.05）。与对照组相比，粘蛋白水平也显著降低（p<0.05）。
慢性摄入甘蔗酒可导致形态学改变，与腺体萎缩相关，降低磷酸酶的生化功能，并减少粘蛋白的合成。
甘蔗酒的摄入可通过改变颌下腺的形态和酶活性来损害其功能。
