Sentell K B, Barnes K W, Dorsey J G
Department of Chemistry, University of Florida, Gainesville 32611.
J Chromatogr. 1988 Nov 25;455:95-104. doi: 10.1016/s0021-9673(01)82109-9.
A reproducible new method for synthesizing high density monomeric reversed-phase chromatographic packings based on the use of ultrasonic waves as the driving force for the bonding reaction is presented. The use of ultrasound has two distinct advantages over traditional reflux methods. It provides a reaction driving force that is independent of temperature; in addition, the power of the ultrasonic driving force can be varied using a variable-power ultrasonic probe. The use of a novel acid-acceptor, 4-dimethylaminopyridine, is also recommended in the bonding reaction due to its ease of use and the resulting high bonding densities.
本文提出了一种可重现的合成高密度单体反相色谱填料的新方法，该方法以超声波作为键合反应的驱动力。与传统回流方法相比，超声波的使用具有两个明显优势。它提供了一个与温度无关的反应驱动力；此外，可使用可变功率超声探头改变超声驱动力的功率。由于其使用方便且能产生高键合密度，在键合反应中还推荐使用一种新型的酸受体——4-二甲氨基吡啶。
