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泰国人群样本中线粒体 DNA 控制区变异。

Mitochondrial DNA control region variation in a population sample from Thailand.

机构信息

Institute of Forensic Medicine, Medical Center - University of Freiburg, Forensic Molecular Biology, Albertstrasse 9, 79104, Freiburg, Germany.

Faculty of Medicine, University of Freiburg, Freiburg, Germany.

出版信息

Int J Legal Med. 2020 Sep;134(5):1563-1568. doi: 10.1007/s00414-020-02303-2. Epub 2020 May 1.

DOI:10.1007/s00414-020-02303-2
PMID:32358724
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7417404/
Abstract

Mitochondrial DNA (mtDNA) control region sequences from hair samples of 213 individuals from Thailand were analyzed using Sanger sequencing. A total of 170 different haplotypes were identified, of which 146 occurred only once (unique haplotypes). The dataset showed a random match probability of 0.87% and a haplotype diversity of 0.9960. The samples were assigned to 85 different haplogroups with B5a, F1a1a, and M being the most frequent ones. Pairwise F-values between this and other Southeast and East Asian populations revealed significant but relatively low differences, indicating a close relation. Heteroplasmic positions were observed in 12.2% of hair samples confirming the frequent appearance of heteroplasmic positions in hairs. This dataset will complement existing data as an mtDNA reference for forensic investigations.

摘要

从泰国的 213 个人的头发样本中进行了线粒体 DNA(mtDNA)控制区序列的 Sanger 测序分析。总共鉴定出 170 种不同的单倍型,其中 146 种仅出现一次(独特单倍型)。该数据集显示随机匹配概率为 0.87%,单倍型多样性为 0.9960。这些样本被分配到 85 个不同的单倍群,其中 B5a、F1a1a 和 M 是最常见的。与其他东南亚和东亚人群的成对 F 值表明存在显著但相对较低的差异,表明存在密切关系。在 12.2%的头发样本中观察到异质位置,证实了异质位置在头发中频繁出现。该数据集将作为 mtDNA 法医调查参考数据的补充。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14c4/7417404/64987c99d6d7/414_2020_2303_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14c4/7417404/64987c99d6d7/414_2020_2303_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14c4/7417404/64987c99d6d7/414_2020_2303_Fig1_HTML.jpg

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本文引用的文献

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Next generation database search algorithm for forensic mitogenome analyses.下一代法医线粒体基因组分析数据库搜索算法。
Forensic Sci Int Genet. 2018 Nov;37:204-214. doi: 10.1016/j.fsigen.2018.09.001. Epub 2018 Sep 9.
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Contrasting maternal and paternal genetic variation of hunter-gatherer groups in Thailand.
对比泰国狩猎采集群体的母系和父系遗传变异。
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Single nucleotide polymorphisms minisequencing in hypervariable regions for screening of Thais.用于泰国人群筛查的高变区单核苷酸多态性微测序技术
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Complete mitochondrial genomes of Thai and Lao populations indicate an ancient origin of Austroasiatic groups and demic diffusion in the spread of Tai-Kadai languages.泰国和老挝人群的完整线粒体基因组表明南亚语系群体的古老起源以及侗台语族传播过程中的人口扩散。
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Mitochondrial control region variation in a Korean population sample.韩国人群样本中线粒体控制区的变异
Int J Legal Med. 2014 Sep;128(5):745-6. doi: 10.1007/s00414-014-0963-7. Epub 2014 Feb 27.
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Large-scale mitochondrial DNA analysis in Southeast Asia reveals evolutionary effects of cultural isolation in the multi-ethnic population of Myanmar.大规模的线粒体 DNA 分析表明,在缅甸多民族群体中,文化隔离对进化产生了影响。
BMC Evol Biol. 2014 Jan 28;14:17. doi: 10.1186/1471-2148-14-17.