RNA 干扰 NADPH-细胞色素 P450 还原酶增加了二斑叶螨对多种杀螨剂的敏感性。
RNA interference of NADPH-cytochrome P450 reductase increases susceptibilities to multiple acaricides in Tetranychus urticae.
机构信息
Irrigated Agriculture Research and Extension Center, Washington State University, Prosser, WA 99350, USA; Department of Entomology, Washington State University, Pullman, WA 99164, USA.
Department of Entomology, Washington State University, Pullman, WA 99164, USA.
出版信息
Pestic Biochem Physiol. 2020 May;165:104550. doi: 10.1016/j.pestbp.2020.02.016. Epub 2020 Feb 28.
The two-spotted spider mite, Tetranychus urticae, is a polyphagous pest feeding on over 1100 plant species, including numerous highly valued economic crops. The control of T. urticae largely depends on the use of acaricides, which leads to pervasive development of acaricide resistance. Cytochrome P450-mediated metabolic detoxification is one of the major mechanisms of acaricide resistance in T. urticae. NADPH-cytochrome P450 reductase (CPR) plays as a crucial co-factor protein that donates electron(s) to microsomal cytochrome P450s to complete their catalytic cycle. This study seeks to understand the involvement of CPR/P450 in acaricide resistance in T. urticae. The full-length cDNA sequence of T. urticae's CPR (TuCPR) was cloned and characterized. TuCPR was ubiquitously transcribed in different life stages of T. urticae and the highest transcription was observed in the nymph and adult stages. TuCPR was constitutively over-expressed in six acaricide resistant populations compared to a susceptible one. TuCPR transcriptional expression was also induced by multiple acaricides in a time-dependent manner. Down-regulation of TuCPR via RNA interference (RNAi) in T. urticae led to reduced enzymatic activities of TuCPR and cytochrome P450s, as well as a reduction of resistance to multiple acaricides, abamectin, bifenthrin, and fenpyroximate. The outcome of this study highlights CPR as a potential novel target for eco-friendly control of T. urticae and other related plant-feeding pests.
二斑叶螨（Tetranychus urticae）是一种多食性害虫，以 1100 多种植物为食，包括许多极具经济价值的作物。T. urticae 的防治在很大程度上依赖于杀螨剂的使用，这导致了杀螨剂抗性的广泛发展。细胞色素 P450 介导的代谢解毒是 T. urticae 对杀螨剂产生抗性的主要机制之一。NADPH-细胞色素 P450 还原酶（CPR）作为一种关键的辅助因子蛋白，将电子（s）供给予微粒体细胞色素 P450 以完成其催化循环。本研究旨在探讨 CPR/P450 在 T. urticae 对杀螨剂抗性中的作用。克隆并鉴定了 T. urticae CPR（TuCPR）的全长 cDNA 序列。TuCPR 在 T. urticae 的不同生命阶段都有广泛的转录，在若虫和成虫阶段转录水平最高。与敏感种群相比，TuCPR 在六个杀螨剂抗性种群中持续过表达。TuCPR 的转录表达也被多种杀螨剂以时间依赖性方式诱导。通过 RNA 干扰（RNAi）下调 T. urticae 中的 TuCPR 表达，导致 TuCPR 和细胞色素 P450s 的酶活性降低，以及对多种杀螨剂（阿维菌素、联苯菊酯和氟吡菌胺）的抗性降低。本研究的结果突出了 CPR 作为一种有潜力的新型目标，可用于生态友好型防治 T. urticae 和其他相关植物取食害虫。
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