The College of Veterinary Medicine, Agricultural University of Hebei, Baoding 071001, China.
The College of Veterinary Medicine, Agricultural University of Hebei, Baoding 071001, China.
Poult Sci. 2020 May;99(5):2757-2765. doi: 10.1016/j.psj.2019.12.053. Epub 2020 Mar 29.
In the present study, for the purpose of investigating the effects of the total flavonoids of Epimedium (TFE) in regard to preventing the development of atrophied oviducts and follicles induced by forced molting, 300-day-old Hy-Line Brown layer hens were divided into 3 study groups as follows: the control (CON) group was the normal group, without forced molting and TFE treatments; the TFE1 group was treated by adding a 1‰ TFE treatment after forced molting; and the TFE0 group was not treated by TFE after forced molting. During this study's experimental process, the egg production rates were recorded each day. In addition, the hens were randomly chosen to be weighed every 4 D and also randomly selected to be sacrificed every 7 D. Then, sample tissues of albumen-secreting part and uterus from the fallopian tube of the layer hens were collected for PCR and hematoxylin-eosin staining tests. The results showed that the body weights, number of follicles, and weights and sizes of the fallopian tube for the TFE1 and TFE0 groups were significantly reduced when compared with those of the control group on the 15th D of the experiment. Furthermore, at the end of study, it was found that the egg production rates, weights of the fallopian tube, and ovarian follicles of TFE1 had recovered to normal levels. At the same time, the serum estrogen and the expressions of the progesterone receptor and estrogen receptor mRNA in fallopian tube were higher than those observed for the TFE0 group. The results of this study provided valuable evidence that TFE could improve the development of atrophied oviducts and increase the egg laying rates, thereby making it a potential multicomponent natural drug for egg production in the future.
在本研究中，为了研究淫羊藿总黄酮（TFE）在预防强制换羽引起的萎缩输卵管和卵泡发育方面的作用，将 300 日龄海兰褐蛋鸡分为 3 个研究组，如下所示：对照组（CON）为正常组，不进行强制换羽和 TFE 处理；TFE1 组在强制换羽后添加 1‰ TFE 处理；TFE0 组在强制换羽后不进行 TFE 处理。在本研究的实验过程中，每天记录产蛋率。此外，每隔 4 天随机选择母鸡称重，每隔 7 天随机选择母鸡处死。然后，收集蛋鸡输卵管蛋白分泌部和子宫的组织样本，进行 PCR 和苏木精-伊红染色试验。结果表明，与对照组相比，实验组第 15 天，TFE1 和 TFE0 组的母鸡体重、卵泡数以及输卵管重量和大小明显减少。此外，在研究结束时，发现 TFE1 组的产蛋率、输卵管重量和卵巢卵泡恢复到正常水平。同时，TFE1 组的血清雌激素水平以及输卵管中孕激素受体和雌激素受体 mRNA 的表达均高于 TFE0 组。本研究结果为 TFE 改善萎缩输卵管的发育和提高产蛋率提供了有价值的证据，使其成为未来生产中潜在的多成分天然产蛋药物。
