Kuźmińska Aleksandra, Butruk-Raszeja Beata A, Stefanowska Aleksandra, Ciach Tomasz
Warsaw University of Technology, Faculty of Chemical and Process Engineering, Biomedical Engineering Laboratory, Warynskiego 1, 00-645 Warsaw, Poland.
Warsaw University of Technology, Faculty of Chemical and Process Engineering, Biomedical Engineering Laboratory, Warynskiego 1, 00-645 Warsaw, Poland.
Colloids Surf B Biointerfaces. 2020 Aug;192:111066. doi: 10.1016/j.colsurfb.2020.111066. Epub 2020 Apr 23.
The presented study describes a method for the preparation and modification of cylindrical polyurethane structures with polyvinylpyrrolidone (PVP) hydrogel coating. The modified polyurethane scaffolds were fabricated using the phase-inversion technique and intended to be used as a vascular prosthesis. The proposed modification method involves a two-step Fenton-type reaction. Physicochemical analysis (Fourier transform infrared spectroscopy, scanning electron microscopy) confirmed the presence of the hydrogel coating. The influence of PVP and polymerization initiator (cumene hydroperoxide) concentrations on hydrogel's properties were examined. The higher concentrations of reagent were used, the thicker coating was obtained. After modification, the material's surface becomes more hydrophilic in comparison to pristine polyurethane. Cytotoxicity assay (MTT test) confirmed that PVP-coating is not toxic. The introduction of hydrogel coating resulted in a significant decrease in the fibrinogen adsorbed to the material's surface as compared to a non-modified polymer. Platelet adhesion assay demonstrated almost no platelet adhesion to the modified surfaces.
本研究描述了一种用聚乙烯吡咯烷酮（PVP）水凝胶涂层制备和改性圆柱形聚氨酯结构的方法。改性聚氨酯支架采用相转化技术制造，旨在用作血管假体。所提出的改性方法涉及两步芬顿型反应。物理化学分析（傅里叶变换红外光谱、扫描电子显微镜）证实了水凝胶涂层的存在。研究了PVP和聚合引发剂（异丙苯过氧化氢）浓度对水凝胶性能的影响。试剂浓度越高，得到的涂层越厚。改性后，与原始聚氨酯相比，材料表面变得更亲水。细胞毒性试验（MTT试验）证实PVP涂层无毒。与未改性聚合物相比，水凝胶涂层的引入导致吸附在材料表面的纤维蛋白原显著减少。血小板粘附试验表明，改性表面几乎没有血小板粘附。
