Waresindo William Xaveriano, Luthfianti Halida Rahmi, Edikresnha Dhewa, Suciati Tri, Noor Fatimah Arofiati, Khairurrijal Khairurrijal
Department of Physics, Faculty of Mathematics and Natural Sciences, Institut Teknologi Bandung Jalan Ganesa 10 Bandung 40132 Indonesia
University Center of Excellence - Nutraceutical, Bioscience and Biotechnology Research Center, Institut Teknologi Bandung Jalan Ganesa 10 Bandung 40132 Indonesia.
RSC Adv. 2021 Sep 9;11(48):30156-30171. doi: 10.1039/d1ra04092h. eCollection 2021 Sep 6.
A polyvinyl alcohol (PVA) hydrogel loaded with guava leaf extract (GLE) has potential applications as a wound dressing with good antibacterial activity. This study succeeded in fabricating a PVA hydrogel containing GLE using the freeze-thaw (FT) method. By varying the GLE concentration, we can adjust the physical properties of the hydrogel. The addition of GLE results in a decrease in cross-linking during gelation and an increase in the pore size of the hydrogels. The increase of the pore size made the swelling increase and the mechanical strength decrease. The weight loss of the hydrogel also increases because the phosphate buffer saline (PBS) dissolves the GLE. Increasing the GLE concentration caused the Fourier-transform infrared (FTIR) absorbance peaks to widen due to hydrogen bonds formed during the FT process. The crystalline phase was transformed into an amorphous phase in the PVA/GLE hydrogel based on the X-ray diffraction (XRD) spectra. The differential scanning calorimetry (DSC) characterization showed a significant decrease in the hydrogel weight over temperatures of 30-150 °C due to the evaporation of water from the hydrogel matrix. The zone of inhibition of the PVA/GLE hydrogel increased with antibacterial activity against of 17.93% per gram and 15.79% per gram against .
负载番石榴叶提取物（GLE）的聚乙烯醇（PVA）水凝胶作为具有良好抗菌活性的伤口敷料具有潜在应用价值。本研究成功采用冻融（FT）法制备了含GLE的PVA水凝胶。通过改变GLE浓度，我们可以调节水凝胶的物理性质。添加GLE导致凝胶化过程中交联减少，水凝胶孔径增大。孔径的增加使溶胀增加而机械强度降低。由于磷酸盐缓冲盐水（PBS）溶解了GLE，水凝胶的重量损失也增加。增加GLE浓度会使傅里叶变换红外（FTIR）吸收峰因冻融过程中形成的氢键而变宽。基于X射线衍射（XRD）光谱，PVA/GLE水凝胶中的晶相转变为非晶相。差示扫描量热法（DSC）表征显示，由于水从水凝胶基质中蒸发，在30 - 150°C温度范围内水凝胶重量显著下降。PVA/GLE水凝胶的抑菌圈随抗菌活性增加，对[具体细菌1]每克为17.93%，对[具体细菌2]每克为15.79%。
