Wang Zhuo, Wang Tietao, Cui Rui, Zhang Zhenxing, Chen Keqi, Li Mengyun, Hua Yueyue, Gu Huawei, Xu Lei, Wang Yao, Yang Yantao, Shen Xihui
State Key Laboratory of Crop Stress Biology for Arid Areas, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Northwest A&F University, Yangling, China.
Front Microbiol. 2020 Apr 17;11:705. doi: 10.3389/fmicb.2020.00705. eCollection 2020.
HpaR, a MarR family transcriptional regulator, was first identified in W for its regulation of the operon. Little else is known regarding its functionality. Here, we report that in , HpaR negatively regulates the operon similar to in W. To investigate additional functions of HpaR, RNA sequencing was performed for both the wild-type and the Δ mutant, which revealed that the type VI secretion system (T6SS) was positively regulated by HpaR. T6SS4 is important for bacteria resisting environmental stress, especially oxidative stress. We demonstrate that HpaR facilitates bacteria resist oxidative stress by upregulating the expression of T6SS4 in . . HpaR is also involved in biofilm formation, antibiotic resistance, adhesion to eukaryotic cells, and virulence in mice. These results greatly expand our knowledge of the functionality of HpaR and reveal a new pathway that regulates T6SS4.
HpaR是一种MarR家族转录调节因子，于2000年首次在W中被鉴定出，因其对操纵子的调节作用。关于其功能，人们所知甚少。在此，我们报道在[具体物种]中，HpaR与在W中一样对操纵子起负调节作用。为了研究HpaR的其他功能，对野生型和Δ突变体进行了RNA测序，结果显示VI型分泌系统（T6SS）受HpaR正调节。T6SS4对于细菌抵抗环境压力，尤其是氧化应激很重要。我们证明HpaR通过上调[具体物种]中T6SS4的表达促进细菌抵抗氧化应激。HpaR还参与生物膜形成、抗生素抗性、对真核细胞的黏附以及在小鼠中的毒力。这些结果极大地扩展了我们对HpaR功能的认识，并揭示了一条调节T6SS4的新途径。
