Ju Yawen, Zhong Rui, Christensen Michael J, Zhang Xingxu
State Key Laboratory of Grassland Agro-ecosystems, Center for Grassland Microbiome, Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, Engineering Research Center of Grassland Industry, Ministry of Education, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, China.
Grasslands Research Centre, AgResearch, Palmerston North, New Zealand.
Front Microbiol. 2020 Apr 17;11:747. doi: 10.3389/fmicb.2020.00747. eCollection 2020.
This study was conducted to explore effects of the systemic fungal endophyte on root and rhizosphere soil bacterial diversity of host plants growing under different moisture conditions. Soil properties of different treatments were compared using standard techniques. A total of 4371379 16S rRNA gene sequences were obtained and assigned to 5025 operational taxonomic units (OTUs). These OTUs in roots and rhizosphere soil were divided into 13 and 17 phyla, respectively, and the Actinobacteria and Proteobacteria were the most abundant phyla both in roots and rhizosphere soil. Shannon diversity and Chao1 richness index of bacteria in rhizosphere soil was significantly higher than in roots. decreased the Shannon diversity of the root-associated bacterial community, and increased Shannon diversity and Chao1 richness index of the rhizosphere soil bacterial community of . Meanwhile, Chao1 richness of the rhizosphere soil bacterial community of significantly increased with the increase of the soil moisture level. Structural equation modeling also emphasized that decreased the diversity of the root-associated bacterial community and increased the diversity of the rhizosphere soil bacterial community through decreasing soil available . Additionally, soil moisture increased the diversity of the rhizosphere soil bacterial community through increased soil pH, C/N, and NN, and decreased soil AP. The endophyte and soil moisture effects on root and rhizosphere soil bacterial diversity were likely to be from responses to modifications of the rhizosphere soil properties.
本研究旨在探讨系统真菌内生菌对在不同水分条件下生长的宿主植物根系和根际土壤细菌多样性的影响。采用标准技术比较不同处理的土壤性质。共获得4371379条16S rRNA基因序列，并将其归为5025个操作分类单元（OTU）。根系和根际土壤中的这些OTU分别分为13个和17个门，放线菌门和变形菌门是根系和根际土壤中最丰富的门。根际土壤细菌的香农多样性和Chao1丰富度指数显著高于根系。[内生菌名称]降低了根际相关细菌群落的香农多样性，并增加了[内生菌名称]根际土壤细菌群落的香农多样性和Chao1丰富度指数。同时，[内生菌名称]根际土壤细菌群落的Chao1丰富度随着土壤水分水平的增加而显著增加。结构方程模型还强调，[内生菌名称]通过降低土壤有效[养分名称]降低了根际相关细菌群落的多样性，并增加了根际土壤细菌群落的多样性。此外，土壤水分通过提高土壤pH值、碳氮比和全氮含量以及降低土壤有效磷含量增加了根际土壤细菌群落的多样性。内生菌和土壤水分对根系和根际土壤细菌多样性的影响可能源于对根际土壤性质变化的响应。
