不同土壤水分条件下内生真菌对表皮蜡质的修饰作用

Cuticular Wax Modification by Endophyte in under Different Soil Moisture Availability.

作者信息

Zhao Zhenrui, Ju Yawen, Kou Mingzhu, Tian Mei, Christensen Michael John, Zhang Xingxu, Nan Zhibiao

机构信息

State Key Laboratory of Grassland Agro-Ecosystems, Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China.

Huaiyin Institute of Agricultural Sciences of Xuhuai Region in Jiangsu, Huai'an 223001, China.

出版信息

J Fungi (Basel). 2022 Jul 12;8(7):725. doi: 10.3390/jof8070725.

DOI:10.3390/jof8070725

PMID:35887480

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9325231/

Abstract

The cuticular wax serves as the outermost hydrophobic barrier of plants against nonstomatal water loss and various environmental stresses. An objective of this study was to investigate the contribution of the mutualistic fungal endophyte to leaf cuticular wax of under different soil moisture availability. Through a pot experiment and gas chromatography-mass spectrometry (GC-MS) analysis, our results indicated that the hydrocarbons were the dominant components of leaf cuticular wax, and the proportion of alcohols, aldehydes, amines, and ethers varied with the presence or absence of and different soil moisture availability. Amines and ethers are unique in endophyte-free (EF) plants and endophyte-infected (EI) plants, respectively. By transcriptome analysis, we found a total of 13 differentially expressed genes (DEGs) related to cuticular biosynthesis, including , , , , , , , , and . A model is proposed which provides insights for understanding cuticular wax biosynthesis in the association of plants with These results may help guide the functional analyses of candidate genes important for improving the protective layer of cuticular wax of endophyte-symbiotic plants.

摘要

角质蜡质作为植物抵御非气孔性水分流失和各种环境胁迫的最外层疏水屏障。本研究的一个目的是调查在不同土壤水分条件下，共生真菌内生菌对叶片角质蜡质的贡献。通过盆栽实验和气相色谱 - 质谱联用（GC - MS）分析，我们的结果表明，烃类是叶片角质蜡质的主要成分，醇类、醛类、胺类和醚类的比例会因内生菌的有无以及不同土壤水分条件而有所变化。胺类和醚类分别在无内生菌（EF）植物和有内生菌感染（EI）植物中具有独特性。通过转录组分析，我们共发现了13个与角质生物合成相关的差异表达基因（DEG），包括、、、、、、、、和。提出了一个模型，该模型为理解植物与共生时的角质蜡质生物合成提供了见解。这些结果可能有助于指导对改善内生菌共生植物角质蜡质保护层重要的候选基因的功能分析。