Zhang Kang, Wang Liming, Si Helong, Guo Hao, Liu Jianhu, Jia Jiao, Su Qianfu, Wang Yanbo, Zang Jinping, Xing Jihong, Dong Jingao
State Key Laboratory of North China Crop Improvement and Regulation, Hebei Agricultural University, Baoding, China.
Hebei Key Laboratory of Plant Physiology and Molecular Pathology, Hebei Agricultural University, Baoding, China.
Front Microbiol. 2022 Oct 20;13:986401. doi: 10.3389/fmicb.2022.986401. eCollection 2022.
Maize stalk rot caused by can reduce the yield of maize and efficiency of mechanized harvesting. Besides, deoxynivalenol and zearalenone toxins produced by can also affect domestic animals and human health. As chemical fungicides are expensive and exert negative effects on the environment, the use of biological control agents has become attractive in recent years. In the present study, we collected rhizosphere soil with severe stalk rot disease (ZDD), the rhizosphere soil with disease-free near by the ZDD (ZDH), and measured rhizosphere microbial diversity and microbial taxonomic composition by amplicon sequencing targeting either bacteria or fungi. The results showed that stalk rot caused by the species among which is frequent and can reduce the abundance and alpha diversity of rhizosphere microbial community, and shift the beta diversity of microorganisms. Furthermore, a bacterial strain, GL-02, isolated from ZDD, was found to significantly affect growth of . and assays demonstrated that GL-02 had good capability to inhibit . These results revealed that GL-02 could be a potential biocontrol agent for the control of maize stalk rot.
由[具体病原菌名称未给出]引起的玉米茎腐病会降低玉米产量和机械化收获效率。此外，[具体病原菌名称未给出]产生的脱氧雪腐镰刀菌烯醇和玉米赤霉烯酮毒素也会影响家畜和人类健康。由于化学杀菌剂价格昂贵且对环境有负面影响，近年来使用生物防治剂变得颇具吸引力。在本研究中，我们采集了患有严重茎腐病的根际土壤（ZDD）、ZDD附近无病的根际土壤（ZDH），并通过针对细菌或真菌的扩增子测序来测定根际微生物多样性和微生物分类组成。结果表明，[具体病原菌名称未给出]引起的茎腐病，其中[具体病原菌种类未给出]较为常见，会降低根际微生物群落的丰度和α多样性，并改变微生物的β多样性。此外，从ZDD中分离出的一株细菌菌株GL - 02被发现对[具体病原菌名称未给出]的生长有显著影响。抑菌和杀菌试验表明GL - 02具有良好的抑制[具体病原菌名称未给出]的能力。这些结果表明GL - 02可能是一种用于防治玉米茎腐病的潜在生物防治剂。
