Cumulative Effects of Constituents from the Mushroom on the Contractility of Penile Corpus Cavernosum Smooth Muscle.

, a puffball mushroom (Agaricaceae), is thought to be an aphrodisiac, as this mushroom is traditionally known to improve sexual function in males. As part of the systematic study to determine the bioactive secondary metabolites from responsible for aphrodisiac effects, chemical analysis of methanol (MeOH) extracts of the fruiting bodies of resulted in the isolation of two major compounds: ,-dimethyl-anthranilic acid () and (7,10)-7,10-octadecadienoic acid methyl ester (). Compounds and were evaluated for cumulative dose-dependent relaxation responses to precontracted penile corpus smooth muscle (PCCSM). Results show that compounds and exhibited a maximum relaxation effect of 20.33 ± 2.18% and 24.63 ± 3.60%, respectively. These findings indicate that compounds and , major components of , could potentially be used to treat erectile dysfunction, functioning as natural aphrodisiacs.