中国成都耐头孢曲松淋病奈瑟菌FC428克隆株的出现及基因组特征分析
Emergence and genomic characterization of the ceftriaxone-resistant Neisseria gonorrhoeae FC428 clone in Chengdu, China.
作者信息
Wang Hongren, Wang Youwei, Yong Gang, Li Xueru, Yu Linchong, Ma Shuaijing, Luo Tao
机构信息
Department of Pathogenic Biology, West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu, China.
Institute of Dermatology and Venereology, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, Chengdu, China.
出版信息
J Antimicrob Chemother. 2020 Sep 1;75(9):2495-2498. doi: 10.1093/jac/dkaa123.
OBJECTIVES
To verify the contribution of the globally disseminated Neisseria gonorrhoeae FC428 clone to the emergence of ceftriaxone resistance in Chengdu in south-west China during 2018.
METHODS
Antimicrobial susceptibility of the N. gonorrhoeae isolates to six antibiotics was determined using the agar dilution method. A real-time PCR assay and WGS were used to identify the FC428 clone. Phylogenomic and molecular antimicrobial resistance analyses were conducted to characterize the transmission and evolution of related strains.
RESULTS
Four out of 112 N. gonorrhoeae isolates were confirmed as the ceftriaxone-resistant FC428 clone. Phylogenomic analysis revealed that they resulted from multiple introductions and subsequent local transmissions. The strains have undergone further evolutions characterized by the accumulation of mutations in resistance-associated genes and/or the acquisition of plasmids encoding penicillin and tetracycline resistance genes.
CONCLUSIONS
The N. gonorrhoeae FC428 clone has spread to south-west China. Efforts should be made to enhance gonococcal antimicrobial surveillance to control further dissemination of this successful clone at both local and national levels.
目的
验证全球传播的淋病奈瑟菌FC428克隆对2018年中国西南部成都地区头孢曲松耐药性出现的影响。
方法
采用琼脂稀释法测定淋病奈瑟菌分离株对六种抗生素的药敏性。采用实时PCR检测和全基因组测序（WGS）鉴定FC428克隆。进行系统基因组学和分子耐药性分析，以表征相关菌株的传播和进化。
结果
112株淋病奈瑟菌分离株中有4株被确认为耐头孢曲松的FC428克隆。系统基因组学分析表明，它们是多次引入并随后在本地传播所致。这些菌株经历了进一步的进化，其特征是耐药相关基因中的突变积累和/或获得编码青霉素和四环素耐药基因的质粒。
结论
淋病奈瑟菌FC428克隆已传播至中国西南部。应努力加强淋球菌抗菌监测，以在地方和国家层面控制这一成功克隆的进一步传播。
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