Dermatology Hospital, Southern Medical University, Lujing Road 2, Yuexiu, Guangzhou, 510091, Guangdong, China.
Guangzhou Key Laboratory for Sexually Transmitted Diseases Control, Guangzhou, Guangdong, People's Republic of China.
Ann Clin Microbiol Antimicrob. 2023 May 17;22(1):39. doi: 10.1186/s12941-023-00587-x.
Antimicrobial resistance (AMR) of untreatable gonococcal infection is an emerging threat, especially in Guangdong, a prosperous province in Southern China.
N.gonorrhoeae was isolated from 20 cities in Guangdong and determined antimicrobial susceptibility. Through whole-genome sequencing (WGS), multilocus sequence typing (MLST), N.gonorrhoeae multiantigen sequence typing (NG-MAST), and N.gonorrhoeae sequence typing for antimicrobial resistance (NG-STAR) were obtained based on the PubMLST database ( https://pubmlst.org/ ). Phylogenetic analysis was used for dissemination and tracking analysis.
Antimicrobial susceptibility was performed on 347 isolates, and 50 isolates were identified as decreased susceptibility (DS) to cephalosporins. Of which 16.0% (8/50) were ceftriaxone DS, 38.0% (19/50) were cefixime DS, and 46.0% (23/50) were both ceftriaxone and cefixime DS. In all, the dual-resistant rate of the cephalosporin-DS isolates was 96.0% for penicillin and 98.0% for tetracycline-resistant, and 10.0% (5/50) were resistant to azithromycin. All cephalosporin-DS isolates were resistant to ciprofloxacin but sensitive to spectinomycin. The predominant MLSTs were ST7363 (16%, 8/50), ST1903 (14%, 7/50), ST1901 (12%, 6/50), and ST7365 (10%, 5/50). Besides some isolates that failed genotyping (NA), NG-STAR ST1143 (n = 6) and NG-MAST ST17748 (n = 4) were the most prevalent. Twelve isolates with mosaic penA-60.001 allele retained the most elevated cephalosporin MIC (Minimum Inhibitory Concentration). Phylogenetic analysis revealed that epidemic penA-60.001 clones, either domestic or foreign, had spread to nine cities in Guangdong, and 9/12 clones were from the Pearl River Delta region.
N. gonorrhoeae with cephalosporins-DS was extensively disseminated in Guangdong, Southern China, requiring strict surveillance.
无法治疗的淋病奈瑟菌感染的抗菌药物耐药性（AMR）是一个新出现的威胁，尤其是在中国南方繁荣的广东省。
从广东省 20 个城市分离淋病奈瑟菌，并测定抗菌药物敏感性。通过全基因组测序（WGS）、多位点序列分型（MLST）、淋病奈瑟菌多抗原序列分型（NG-MAST）和淋病奈瑟菌抗菌药物耐药性序列分型（NG-STAR），基于 PubMLST 数据库（https://pubmlst.org/）进行检测。采用系统发育分析进行传播和追踪分析。
对 347 株分离株进行了抗菌药物敏感性检测，其中 50 株对头孢菌素表现出低敏性（DS）。其中，16.0%（8/50）为头孢曲松 DS，38.0%（19/50）为头孢克肟 DS，46.0%（23/50）为头孢曲松和头孢克肟 DS。所有头孢菌素-DS 分离株对青霉素的双重耐药率为 96.0%，对四环素的耐药率为 98.0%，对阿奇霉素的耐药率为 10.0%（5/50）。所有头孢菌素-DS 分离株均对环丙沙星耐药，但对壮观霉素敏感。主要的 MLST 型别为 ST7363（16%，8/50）、ST1903（14%，7/50）、ST1901（12%，6/50）和 ST7365（10%，5/50）。除了一些未进行基因分型的分离株（NA）外，NG-STAR ST1143（n=6）和 NG-MAST ST17748（n=4）是最常见的。携带 mosaic penA-60.001 等位基因的 12 株分离株保留了最高的头孢菌素 MIC（最低抑菌浓度）。系统发育分析显示，来自国内外的流行的 penA-60.001 克隆已传播到广东省的 9 个城市，其中 9 个克隆来自珠江三角洲地区。
中国南方广东省广泛传播的头孢菌素-DS 淋病奈瑟菌需要严格监测。
