Centro de Estudios Parasitológicos y Vectores (CEPAVE), Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad Nacional de La Plata (CCT, CONICET-UNLP), calle 120 s/n e/60 y 64, 1900, La Plata, Buenos Aires, Argentina.
Laboratorio de Histología y Embriología Descriptiva, Experimental y Comparada, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, Av. 60 y 118 s/n., La Plata, Buenos Aires, Argentina.
J Helminthol. 2020 May 4;94:e148. doi: 10.1017/S0022149X20000292.
In the Laboratory of Parasites of Fishes, Crustaceans and Mollusks (CEPAVE), we undertook a parasitological study on three species of fish from the Espinal and Esteros del Iberá ecoregions of Argentina. Clinostomid metacercariae were found parasitizing Characidium rachovii, Crenicichla vittata and Gymnogeophagus balzanii. In this study, we analysed the damage that these parasites inflict on their hosts through the evaluation of histological sections. In addition, Clinostomum metacercariae were identified using morphological characters and DNA barcoding. In the pathological analysis, we observed that muscle tissue was the most affected. The inflammatory response showed vascular congestion areas and infiltration of numerous inflammatory cells, mainly lymphocytes. The molecular and morphological approach supports the presence of three new lineages of clinostomid metacercariae in Argentina. This could lead to the discovery of a high number of lineages or species of Clinostomum from South America.
在鱼类、甲壳类和软体动物寄生虫实验室（CEPAVE），我们对来自阿根廷埃斯皮纳尔和伊贝拉埃斯特罗斯生态区的三种鱼类进行了寄生虫学研究。在克拉西多米幼虫中发现了寄生在拉乔维氏鱼、维塔塔氏石首鱼和巴兰氏琴脂鲤身上的寄生幼虫。在这项研究中，我们通过评估组织切片分析了这些寄生虫对宿主造成的损害。此外，我们还使用形态特征和 DNA 条形码对克拉西多米幼虫进行了鉴定。在病理学分析中，我们观察到肌肉组织是受影响最严重的组织。炎症反应显示血管充血区和大量炎症细胞浸润，主要是淋巴细胞。分子和形态学方法支持在阿根廷存在三种新的克拉西多米幼虫谱系。这可能会发现大量来自南美的 Clinostomum 谱系或物种。
