Yang Yanhui, Zhang Zhongyi, Li Ruifang, Yi Yanjie, Yang Heng, Wang Chaojie, Wang Zushiqi, Liu Yunyi
College of Bioengineering, Henan University of Technology, Lianhua Street 100, Zhengzhou High-Technology Zero, Zhengzhou 450001, China.
College of Crop Sciences, Fujian Agriculture and Forestry University, Jinshan Road, Cangshan District, Fuzhou 350002, China.
Plants (Basel). 2020 Apr 29;9(5):567. doi: 10.3390/plants9050567.
production is affected by replanting disease, in which autotoxic harm to plants is mediated by endogenous phenolic acids as allelopathic compounds found in root exudates. These phenolic acids are mostly phenylpropanoid products of plants' secondary metabolisms. The molecular mechanism of their biosynthesis and release has not been explored in . -coumarate-3-hydroxylase (C3H) is the second hydroxylase gene involved in the phenolic acid/phenylpropanoid biosynthesis pathways. C3Hs have been functionally characterized in several plants. However, limited information is available on the gene in . Here, we identified a putative gene and predicted its potential function by in silico analysis and subcellular localization. Overexpression or repression of in the transgenic roots indicated that the gene was involved in allelopathic phenolic biosynthesis. Moreover, we found that these phenolic acid release amount of the transgenic roots were altered, implying that positively promotes their release via the molecular networks of the activated phenolic acid/phenylpropanoid pathways. This study revealed that plays roles in the biosynthesis and release of allelopathic phenolic acids in roots, laying a basis for further clarifying the molecular mechanism of the replanting disease development.
作物产量受到连作障碍的影响，在连作障碍中，植物的自毒危害是由作为化感物质的内源酚酸介导的，这些酚酸存在于根系分泌物中。这些酚酸大多是植物次生代谢的苯丙烷类产物。其生物合成和释放的分子机制在[具体植物名称未给出]中尚未得到探索。对香豆酸-3-羟化酶（C3H）是参与酚酸/苯丙烷类生物合成途径的第二个羟化酶基因。C3H在几种植物中已有功能表征。然而，关于[具体植物名称未给出]中该基因的信息有限。在这里，我们鉴定了一个假定的[具体植物名称未给出]C3H基因，并通过电子分析和亚细胞定位预测了其潜在功能。在转基因[具体植物名称未给出]根系中过表达或抑制该基因表明该基因参与化感酚类物质的生物合成。此外，我们发现转基因[具体植物名称未给出]根系中这些酚酸释放量发生了改变，这意味着[具体植物名称未给出]C3H通过激活的酚酸/苯丙烷类途径的分子网络正向促进它们的释放。本研究揭示了[具体植物名称未给出]C3H在[具体植物名称未给出]根系化感酚酸的生物合成和释放中发挥作用，为进一步阐明连作障碍发生的分子机制奠定了基础。
