College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
Key Laboratory of Viticulture and Enology, Ministry of Agriculture and Rural Affairs, Beijing 100083, China.
Genes (Basel). 2020 Apr 29;11(5):485. doi: 10.3390/genes11050485.
Glycosylated volatile precursors are important, particularly in wine grape berries, as they contribute to the final aroma in wines by releasing volatile aglycones during yeast fermentation and wine storage. Previous study demonstrated that VviGT14 was functioned as a critical monoterpene glucosyltransferase in grape berry, while the transcriptional regulation mechanism of was still unknown. Here we identified VviWRKY40 as a binding factor of promoter by both DNA pull-down and yeast one-hybrid screening, followed by a series of in vitro verification. expression pattern negatively correlated with that of in grape berries. And the suppressor role of VviWRKY40 was further confirmed by using the dual luciferase assay with Arabidopsis protoplast and grape cell suspension system. Furthermore, the grape suspension cell ABA treatment study showed that ABA downregulated transcript level but promoted that of , indicating that VviWRKY40 was at the downstream of ABA signal transduction network to regulate monoterpenoid glycosylation. These data extend our knowledge of transcriptional regulation of , and provide new targets for grape breeding to alter monoterpenoid composition.
糖基化挥发性前体物很重要,特别是在酿酒葡萄浆果中,因为它们在酵母发酵和葡萄酒储存过程中释放出挥发性糖苷配基,从而有助于葡萄酒的最终香气。先前的研究表明,VviGT14 在葡萄浆果中作为一种关键的单萜葡萄糖基转移酶发挥作用,而 的转录调控机制尚不清楚。在这里,我们通过 DNA 下拉和酵母单杂交筛选鉴定 VviWRKY40 为 启动子的结合因子,随后进行了一系列体外验证。在葡萄浆果中, 的表达模式与 的表达模式呈负相关。并且通过使用双荧光素酶测定法和拟南芥原生质体和葡萄细胞悬浮系统进一步证实了 VviWRKY40 的抑制作用。此外,葡萄悬浮细胞 ABA 处理研究表明,ABA 下调 转录水平,但促进 ,表明 VviWRKY40 位于 ABA 信号转导网络的下游,以调节单萜糖苷化。这些数据扩展了我们对 的转录调控的认识,并为改变单萜类化合物组成的葡萄育种提供了新的目标。