Division of Urology, China Medical University and Hospital, Taichung, Taiwan, R.O.C.
Sex Hormone Research Center and Department of Laboratory Medicine and Biotechnology, China Medical University and Hospital, Taichung, Taiwan, R.O.C.
Anticancer Res. 2020 May;40(5):2715-2724. doi: 10.21873/anticanres.14243.
BACKGROUND/AIM: Interactions between stromal and tumor cells in tumor microenvironment contribute to tumor progression. In bladder cancer (BCa), infiltration of macrophages in tumors correlates with cancer progression. Herein, the aim was to study the paracrine effects of tumor-associated macrophages (TAM) on BCa cells.
The correlation between TAMs and tumor grade and stages was examined in tumor tissue microarrays. In addition, a conditioned media (CM) model was employed to investigate the paracrine effects of macrophages on BCa cell growth, migration, and invasion, as well as on the cytokine profile of each cell line.
The correlation of tumor-infiltrating macrophages with high-grade and muscle-invasive BCa was demonstrated in human bladder tumor tissue microarrays. CM from co-cultures of macrophages and BCa cells increased BCa cell growth, migration and invasion. Moreover, higher mRNA and protein expression levels of CCL5 and IL-8 were found in cells and CM from co-cultures, respectively.
The paracrine interaction between BCa cells and TAMs led to enhanced BCa cell growth, migration, and invasiveness, and moreover, increased IL-8 and CCL5 cytokine production in tumor microenvironment.
背景/目的：肿瘤微环境中基质细胞与肿瘤细胞的相互作用促进了肿瘤的进展。在膀胱癌（BCa）中，肿瘤内巨噬细胞的浸润与癌症进展相关。在此，旨在研究肿瘤相关巨噬细胞（TAM）对 BCa 细胞的旁分泌作用。
在肿瘤组织微阵列中检查 TAM 与肿瘤分级和分期之间的相关性。此外，还采用了条件培养基（CM）模型来研究巨噬细胞对 BCa 细胞生长、迁移和侵袭的旁分泌作用，以及对每种细胞系细胞因子谱的影响。
在人类膀胱癌组织微阵列中证实了浸润性肿瘤巨噬细胞与高级别和肌层浸润性 BCa 之间的相关性。巨噬细胞和 BCa 细胞共培养的 CM 增加了 BCa 细胞的生长、迁移和侵袭。此外，共培养细胞和 CM 中的 CCL5 和 IL-8 的 mRNA 和蛋白表达水平均升高。
BCa 细胞与 TAMs 之间的旁分泌相互作用导致 BCa 细胞生长、迁移和侵袭能力增强，并增加了肿瘤微环境中 IL-8 和 CCL5 细胞因子的产生。
