在食管鳞状细胞癌中发挥致癌作用。

Plays an Oncogenic Role in Esophageal Squamous Cell Carcinoma.

机构信息

Department of Surgery, Kyushu University Beppu Hospital, Oita, Japan.

Department of Gastroenterological Surgery, Graduate School of Medicine, Osaka University, Osaka, Japan.

出版信息

Anticancer Res. 2020 May;40(5):2941-2946. doi: 10.21873/anticanres.14272.

DOI:10.21873/anticanres.14272

PMID:32366446

Abstract

BACKGROUND/AIM: The spliceosome pathway, including Splicing Factor 3b Subunit 4 (SF3B4), plays an important role in carcinogenesis and progression in various cancers; however, the clinical relevance of SF3B4 in esophageal squamous cell carcinoma (ESCC) remains unknown.

PATIENTS AND METHODS

SF3B4 expression was evaluated by real-time reverse transcription polymerase chain reaction in 80 ESCC patients. In order to explore the mechanism of SF3B4 in ESCC, the mRNA expression and copy number of SF3B4 were obtained from TCGA and we also implemented gene set enrichment analysis (GSEA).

RESULTS

The high SF3B4 expression group (n=33) showed significantly more lymphatic permeation and poorer prognosis than the low SF3B4 expression group (n=47). GSEA revealed that high SF3B4 expression was correlated with genes associated with the transcription factor E2F and the G/M checkpoint. SF3B4 expression was positively correlated with SF3B4 DNA copy number.

CONCLUSION

Over-expression of SF3B4 may play a crucial role in the lymphatic progression of ESCC.

摘要

背景/目的：剪接体途径，包括剪接因子 3b 亚基 4（SF3B4），在多种癌症的发生和进展中发挥着重要作用；然而，SF3B4 在食管鳞状细胞癌（ESCC）中的临床相关性尚不清楚。

患者和方法

通过实时逆转录聚合酶链反应（real-time reverse transcription polymerase chain reaction）在 80 例 ESCC 患者中评估 SF3B4 的表达。为了探索 SF3B4 在 ESCC 中的作用机制，我们从 TCGA 获得了 SF3B4 的 mRNA 表达和拷贝数，并进行了基因集富集分析（gene set enrichment analysis，GSEA）。