Botany and Microbiology Department, Faculty of Science, Zagazig University, Zagazig, Egypt.
J Basic Microbiol. 2020 Aug;60(8):712-721. doi: 10.1002/jobm.202000087. Epub 2020 May 4.
The benefits of growth-stimulating microbes in crop production represent great opportunities for recent agricultural practices. Thus, the present investigation deals with examining whether arbuscular mycorrhizal (AM) fungi or Trichoderma viride application or their dual inoculation could improve the biochemical parameters and mineral and nutrient contents of onion plants (Allium cepa) under glasshouse conditions. The results evidenced that both AM fungi and T. viride are compatible with each other, and their combined use is effective, not only in improving the biochemical parameters, such as total soluble carbohydrates, protein contents, total free amino acids, acid, and alkaline phosphatases, but also in increasing mineral and nutrient contents (N, P, K , Ca , Mg , and Zn) in onion plants, in which an increase of 67%, 49%, and 112% was observed in shoot onion P content with AM, T. viride, and with their dual inoculation, respectively, as compared with the controlled ones. Also, AM fungal colonization percentage augmented greatly with T. viride inoculation. Moreover, the protein profile of onion leaves revealed the appearance of newly protein bands with AM and T. viride applications. Therefore, their applications improved onion plant development, which could be used to replace the expensive chemical fertilizers, thus increasing onion quality.
在作物生产中，促进生长的微生物的益处为现代农业实践带来了巨大的机遇。因此，本研究旨在探讨丛枝菌根（AM）真菌或木霉（Trichoderma viride）的应用，或它们的双重接种是否可以改善温室条件下洋葱（Allium cepa）植物的生化参数以及矿物质和养分含量。结果表明，AM 真菌和木霉相互兼容，并且它们的联合使用不仅有效提高了生化参数，如总可溶性碳水化合物、蛋白质含量、总游离氨基酸、酸和碱性磷酸酶，而且还增加了洋葱植物的矿物质和养分（N、P、K、Ca、Mg 和 Zn）含量，与对照相比，AM、木霉及其双重接种分别使洋葱地上部磷含量增加了 67%、49%和 112%。此外，AM 真菌的定殖百分比随着木霉的接种而大大增加。此外，洋葱叶片的蛋白质图谱显示，AM 和木霉的应用出现了新的蛋白质条带。因此，它们的应用改善了洋葱植物的发育，可以用来替代昂贵的化学肥料，从而提高洋葱的品质。
