Szymanowicz Joanna, Schwarz Tomasz, Murawski Maciej, Małopolska Martyna, Oszczęda Zdzisław, Tuz Ryszard, Nowicki Jacek, Bartlewski Pawel Mieczyslaw
Agricultural University of Cracow, Department of Animal Biotechnology, Kraków, Poland.
Agricultural University of Cracow, Department of Swine and Small Animal Breeding, Kraków, Poland.
Anim Reprod. 2019 Nov 18;16(4):864-870. doi: 10.21451/1984-3143-AR2019-0023.
The aim of this study was to assess the usefulness of nanowater (NW; water declusterized using cold plasma treatment) as a diluent for a commercial boar semen extender during the 15-day storage (Days 1 to 15) at 16-18 °C. Ejaculates collected from 8 boars were subjected to the standard evaluation and then diluted in the extender prepared with deionized water (DW) or NW to a final concentration of 3×10 spermatozoa/ml. The proportion of defective spermatozoa increased (P<0.05) from Day 10 to Day 15 of storage (22.8±16.6% to 41.8±26.4% in DW group and 18.6±11.7% to 34.8±25.4% in NW group) and it was significantly greater in DW group compared with NW group on Days 5 and 10 due mainly to a greater (P<0.05) number of mid-piece defects in semen stored in the DW-containing extender. Sperm progressive motility decreased (P<0.05) in both groups between Days 2 and 6, Days 6 and 10, and Days 10 and 12, whereas the percentage of motile spermatozoa declined (P<0.05) to Day 14 only in NW group. Sperm motility was greater (P<0.05) in NW group compared with DW group from Day 5 to Day 13. A decline in sperm progressive motility below 40% in all semen samples occurred by Day 11 in DW group and by Day 12 in NW group. The mean survival time of sperm at 37 °C was greater in NW group than in DW group on Day 5 (314±87 min compared with 284±87 min) and Day 10 (223±34 min compared with 182±27 min; NW group compared with DW group, respectively). There were no differences (P>0.05) between the two groups in the concentrations of alkaline phosphatase and aspartate aminotransferase in semen extender. To summarize, the use of NW as an extender diluent exerts cytoprotective effects on boar spermatozoa and delays a decline in sperm progressive motility.
本研究的目的是评估纳米水（NW；使用冷等离子体处理使水去簇化）作为商业公猪精液稀释剂在16 - 18°C下储存15天（第1天至第15天）期间的效用。从8头公猪采集的射精样本进行标准评估，然后用去离子水（DW）或NW制备的稀释剂稀释至最终浓度为3×10个精子/毫升。储存第10天至第15天，有缺陷精子的比例增加（P<0.05）（DW组从22.8±16.6%增至41.8±26.4%，NW组从18.6±11.7%增至34.8±25.4%），并且在第5天和第10天，DW组该比例显著高于NW组，这主要是因为储存在含DW稀释剂中的精液中段缺陷数量更多（P<0.05）。两组在第2天和第6天、第6天和第10天以及第10天和第12天之间精子的前向运动性均下降（P<0.05），而仅NW组中活动精子的百分比在第14天下降（P<0.05）。从第5天至第13天，NW组的精子运动性高于DW组（P<0.05）。DW组在第11天、NW组在第12天，所有精液样本中精子的前向运动性均降至40%以下。在第5天（NW组为314±87分钟，DW组为284±87分钟）和第10天（NW组为223±34分钟，DW组为182±27分钟；NW组与DW组分别比较），NW组精子在37°C下的平均存活时间长于DW组。两组精液稀释剂中碱性磷酸酶和天冬氨酸转氨酶的浓度无差异（P>0.05）。总之，使用NW作为稀释剂对公猪精子具有细胞保护作用，并延缓精子前向运动性的下降。
