Plant Physiology Section, Department of Evolutionary Biology, Ecology and Environmental Sciences, Faculty of Biology, University of Barcelona, Diagonal 643, Barcelona, 08028, Spain.
AGROTECNIO (Center for Research in Agrotechnology), Av. Rovira Roure 191, Lleida, 25198, Spain.
Plant J. 2020 Aug;103(4):1603-1613. doi: 10.1111/tpj.14799. Epub 2020 Jun 14.
In wheat (Triticum aestivum L) and other cereals, the number of ears per unit area is one of the main yield-determining components. An automatic evaluation of this parameter may contribute to the advance of wheat phenotyping and monitoring. There is no standard protocol for wheat ear counting in the field, and moreover it is time consuming. An automatic ear-counting system is proposed using machine learning techniques based on RGB (red, green, blue) images acquired from an unmanned aerial vehicle (UAV). Evaluation was performed on a set of 12 winter wheat cultivars with three nitrogen treatments during the 2017-2018 crop season. The automatic system uses a frequency filter, segmentation and feature extraction, with different classification techniques, to discriminate wheat ears in micro-plot images. The relationship between the image-based manual counting and the algorithm counting exhibited high levels of accuracy and efficiency. In addition, manual ear counting was conducted in the field for secondary validation. The correlations between the automatic and the manual in-situ ear counting with grain yield were also compared. Correlations between the automatic ear counting and grain yield were stronger than those between manual in-situ counting and GY, particularly for the lower nitrogen treatment. Methodological requirements and limitations are discussed.
在小麦(Triticum aestivum L)和其他谷物中,单位面积的穗数是主要产量决定因素之一。该参数的自动评估可能有助于推进小麦表型和监测。目前在田间还没有小麦穗数计数的标准方案,而且该过程非常耗时。本研究提出了一种基于机器视觉的自动穗数计数系统,该系统使用基于从无人机获取的 RGB(红、绿、蓝)图像的机器学习技术。在 2017-2018 作物季,对 12 个冬小麦品种在三种氮素处理下进行了评估。自动系统使用频率滤波器、分割和特征提取,并结合不同的分类技术,以区分微型小区图像中的小麦穗。基于图像的手动计数与算法计数之间的关系表现出高精度和高效率。此外,还在田间进行了手动穗数的二次验证。还比较了自动和手动田间穗数计数与籽粒产量之间的关系。自动穗数计数与籽粒产量之间的相关性强于手动田间计数与 GY 的相关性,特别是在较低氮素处理下。讨论了方法学的要求和局限性。