Department of Microbiology, School of Medicine, Fukushima Medical University, Fukushima 960-1295, Japan.
Nutrients. 2020 May 1;12(5):1285. doi: 10.3390/nu12051285.
Various berries demonstrate antioxidant activity, and this effect is expected to prevent chronic diseases. We examined whether a diet containing blueberry powder could prevent the development of bladder dysfunction secondary to bladder outlet obstruction (BOO). Eighteen 8-week-old male Sprague-Dawley rats were randomly divided into three groups: Sham (sham operated + normal diet), N-BOO (BOO operated + normal diet) and B-BOO (BOO operated + blueberry diet). Four weeks after BOO surgery, the N-BOO group developed bladder dysfunction with detrusor overactivity. The B-BOO group showed significantly improved micturition volume and micturition interval. The urinary levels of 8-hydroxy-2'-deoxyguanosine (8-OHdG) and malondialdehyde (MDA) were measured as oxidative stress markers. In the N-BOO group, 8-OHdG increased 1.6-fold and MDA increased 1.3-fold at 4 weeks after surgery, whereas the increase in 8-OHdG was significantly reduced by 1.1-fold, despite a similar increase in MDA, in the B-BOO group. Bladder remodeling was confirmed due to bladder hypertrophy, fibrosis and increased connexin43 expression in the N-BOO group, but these histological changes were reduced in the B-BOO group. The intake of blueberries prevented the development of bladder dysfunction secondary to BOO. This effect seems to be related to antioxidation and the inhibition of bladder remodeling.
各种浆果都具有抗氧化活性，这种作用有望预防慢性疾病。我们研究了蓝莓粉饮食是否可以预防因膀胱出口梗阻（BOO）引起的膀胱功能障碍的发展。将 18 只 8 周龄雄性 Sprague-Dawley 大鼠随机分为三组：Sham（假手术+普通饮食）、N-BOO（BOO 手术+普通饮食）和 B-BOO（BOO 手术+蓝莓饮食）。BOO 手术后 4 周，N-BOO 组出现逼尿肌过度活动的膀胱功能障碍。B-BOO 组的排尿量和排尿间隔明显改善。测量尿液中的 8-羟基-2'-脱氧鸟苷（8-OHdG）和丙二醛（MDA）作为氧化应激标志物。在 N-BOO 组中，手术后 4 周时 8-OHdG 增加了 1.6 倍，MDA 增加了 1.3 倍，而 B-BOO 组中 8-OHdG 增加了 1.1 倍，尽管 MDA 增加相似。由于膀胱肥大、纤维化和连接蛋白 43 表达增加，N-BOO 组确认了膀胱重塑，但在 B-BOO 组中这些组织学变化减少了。蓝莓的摄入可预防 BOO 引起的膀胱功能障碍。这种作用似乎与抗氧化作用和抑制膀胱重塑有关。
