The present study reports the synthesis, characterization, and crystal structure of a novel bioactive metal-organic framework, [Ag(-PTA)-PTA)(-pma)(HO)]·6nHO (bioMOF ), which was assembled from silver(I) oxide, 1,3,5-triaza-7-phosphaadamantane (PTA), and pyromellitic acid (Hpma). This product was isolated as a stable microcrystalline solid and characterized by standard methods, including elemental analysis, H and P{H} NMR and FTIR spectroscopy, and single crystal X-ray diffraction. The crystal structure of disclosed a very complex ribbon-pillared 3D metal-organic framework driven by three different types of bridging ligands (-PTA, -PTA, and -pma). Various bioactivity characteristics of bioMOF were investigated, revealing that this compound acts as a potent antimicrobial against pathogenic strains of standard Gram-negative (, ) and Gram-positive () bacteria, as well as a yeast (). Further, showed significant antiviral activity against human adenovirus 36 (HAdV-36). Finally, bioMOF revealed high cytotoxicity toward an abnormal epithelioid cervix carcinoma (HeLa) cell line with low toxicity toward a normal human dermal fibroblast (NHDF) cell line. This study not only broadens the family of PTA-based coordination polymers but also highlights their promising multifaceted bioactivity.