Nisyrios Themistoklis, Karygianni Lamprini, Fretwurst Tobias, Nelson Katja, Hellwig Elmar, Schmelzeisen Rainer, Al-Ahmad Ali
Department of Oral and Craniomaxillofacial Surgery, Center for Dental Medicine, University Medical Center Freiburg, Faculty of Medicine, 79106 Freiburg, Germany.
Clinic for Preventive Dentistry, Periodontology and Cariology, Center for Dental Medicine, University of Zurich, 8032 Zurich, Switzerland.
Materials (Basel). 2020 May 1;13(9):2102. doi: 10.3390/ma13092102.
Bone graft infections represent a challenge in daily clinics, resulting in increased patient discomfort and graft removal. The aim of this study was to investigate the initial adhesion of five representative pathogens on three different block bone graft materials (xenogeneic, alloplastic and allogeneic) and to assess if chlorhexidine (CHX) can effectively control the initial bacterial adhesion. Three different block bone grafting materials (Tutobone®, Endobon® and human spongiosa) were incubated with , , , and in the presence or absence of 0.2% CHX solution. Bacterial adhesion was assessed by the direct counting of the colony-forming units (CFUs) and visualized by scanning electron microscopy (SEM). Overall, the selected bacterial species adhered successfully to all tested bone replacement scaffolds, which showed similar bacterial counts. The lg CFU values ranged from 5.29 ± 0.14 to 5.48 ± 0.72 for , from 4.37 ± 0.62 to 5.02 ± 0.48 for , from 4.92 ± 0.34 to 4.95 ± 0.21 for , from 4.97 ± 0.40 to 5.22 ± 0.13 for and from 4.23 ± 0.54 to 4.58 ± 0.26 for CHX did not interfere with initial microbial adhesion, and yet it killed all adhered bacterial cells. Thus, CHX can be used to prevent subsequent biofilm infections.
骨移植感染是日常临床工作中的一项挑战，会导致患者不适加剧以及移植骨被移除。本研究的目的是调查五种代表性病原体在三种不同块状骨移植材料（异种骨、人工合成骨和同种异体骨）上的初始黏附情况，并评估洗必泰（CHX）是否能有效控制细菌的初始黏附。将三种不同的块状骨移植材料（Tutobone®、Endobon®和人松质骨）在有或没有0.2% CHX溶液存在的情况下，与金黄色葡萄球菌、大肠埃希菌、铜绿假单胞菌、粪肠球菌和白色念珠菌一起孵育。通过直接计数菌落形成单位（CFU）来评估细菌黏附情况，并通过扫描电子显微镜（SEM）进行可视化观察。总体而言，所选细菌种类成功黏附于所有测试的骨替代支架，且细菌计数相似。金黄色葡萄球菌的lg CFU值范围为5.29±0.14至5.48±0.72，大肠埃希菌为4.37±0.62至5.02±0.48，铜绿假单胞菌为4.92±0.34至4.95±0.21，粪肠球菌为4.97±0.40至5.22±0.13，白色念珠菌为4.23±0.54至4.58±0.26。CHX并不干扰微生物的初始黏附，但它能杀死所有黏附的细菌细胞。因此，CHX可用于预防随后的生物膜感染。
