Department of Surgical, Oncological and Oral Sciences, University of Palermo, Palermo, Italy.
Department of Health Promotion Sciences and Mother-Child Care "G. D'Alessandro", University of Palermo, Palermo, Italy.
Biomed Res Int. 2017;2017:2175019. doi: 10.1155/2017/2175019. Epub 2017 Dec 20.
The aim of this study was to evaluate if antibiotic prophylaxis reduces the bacterial contamination of bone particles collected directly from the burs used for implant site preparation. Thirty-four patients underwent the surgical procedures for a total of 34 implant sites. One 1 gr. tablet of amoxicillin + clavulanic acid was given to the test group 12 hours and 1 hour before the surgery. The control group did not take antibiotic prophylaxis. Bone particles were collected and centrifuged. The suspensions were subjected to serial dilutions and each dilution was examined twice using a spatulation technique in Trypticase Soy Agar (TSA), in Sabouraud Dextrose Agar, and in Mitis Salivarius Agar (MSA). The number of colonies was calculated and the identification of various microorganisms was made. The most represented species, in both groups of patients, belonged to the "oral Streptococci." For TSA, the test and control groups differed significantly ( = 0.018). Conversely, there was no significant difference for MSA ( = 0.201) and for the number of bacterial species isolated in the samples of the two groups of patients ( = 0.898). The antibiotic prophylaxis reduced, but did not cancel, the risk of infection of the autogenous particulate bone graft. This trial is registered with IRCT2017102537002N1.
本研究旨在评估抗生素预防是否能降低从用于种植体预备的冲洗液中直接采集的骨颗粒的细菌污染。34 名患者接受了总共 34 个种植体部位的手术。实验组在手术前 12 小时和 1 小时给予 1 克阿莫西林+克拉维酸片。对照组未接受抗生素预防。采集骨颗粒并离心。将悬浮液进行连续稀释，然后使用涂抹技术在胰蛋白酶大豆琼脂（TSA）、沙氏葡萄糖琼脂（SDA）和米氏链球菌琼脂（MSA）中检查每种稀释液两次。计算菌落数量，并对各种微生物进行鉴定。两组患者中最常见的物种均属于“口腔链球菌”。对于 TSA，实验组和对照组之间存在显著差异（=0.018）。相反，对于 MSA（=0.201）和两组患者样本中分离的细菌种类数量（=0.898），差异无统计学意义。抗生素预防降低了，但并未消除，自体颗粒骨移植物感染的风险。该试验在 IRCT2017102537002N1 注册。
