Krzyżek Paweł, Pawełka Dorota, Iwańczak Barbara, Kempiński Radosław, Leśniakowski Konrad, Mégraud Francis, Łaczmański Łukasz, Biernat Monika, Gościniak Grażyna
Department of Microbiology, Wroclaw Medical University, 50-368 Wroclaw, Poland.
Department and Division of Surgical Didactics, Wroclaw Medical University, 50-368 Wroclaw, Poland.
Antibiotics (Basel). 2020 May 2;9(5):228. doi: 10.3390/antibiotics9050228.
Monitoring the antibiotic resistance of is an important step in the effective treatment of this bacterium, thus the aim of the present study was to assess the prevalence of antimicrobial resistance of strains isolated from pediatric and adult patients with primary infections in 2016-2018. Antral biopsies from 334 treatment-naïve patients (126 children and 208 adults) were obtained. A total of 71 clinical strains (22 from children and 49 from adults) were isolated and examined for amoxicillin (AMX), clarithromycin (CLR), metronidazole (MTZ), tetracycline (TET), and levofloxacin (LEV) susceptibility. The activity of the antibiotics was measured by E-tests. Strains were considered as resistant to antibiotics with minimum inhibitory concentrations (MICs) equal to ≥0.125 μg/mL (AMX), ≥0.5 μg/mL (CLR), ≥8 μg/mL (MTZ), and ≥1 μg/mL (TET and LEV). The highest prevalence of antibiotic resistance in strains was observed for CLR and MTZ, at frequencies of 54.5% and 31.8% vs. 30.6% and 46.9% for children and adults, respectively. A much lower frequency of isolation of resistant strains was demonstrated for LEV and TET, this being 9.1% and 4.5% vs. 18.4% and 4.1% for pediatric and adult patients, respectively. The presence of AMX-resistant strains was not observed. The strains isolated from Polish patients with primary infections showed a high level of antibiotic resistance to CLR and MTZ (>30%).
监测[细菌名称]的抗生素耐药性是有效治疗该细菌的重要一步，因此本研究的目的是评估2016 - 2018年从患有原发性感染的儿科和成人患者中分离出的[细菌名称]菌株的抗菌药物耐药性流行情况。从334例未接受过治疗的患者（126名儿童和208名成人）获取了鼻窦活检样本。共分离出71株临床[细菌名称]菌株（22株来自儿童，49株来自成人），并检测了它们对阿莫西林（AMX）、克拉霉素（CLR）、甲硝唑（MTZ）、四环素（TET）和左氧氟沙星（LEV）的敏感性。通过E试验测定抗生素的活性。当最低抑菌浓度（MIC）等于≥0.125μg/mL（AMX）、≥0.5μg/mL（CLR）、≥8μg/mL（MTZ）以及≥1μg/mL（TET和LEV）时，菌株被视为对相应抗生素耐药。在[细菌名称]菌株中，观察到对CLR和MTZ的抗生素耐药率最高，儿童和成人的耐药频率分别为54.5%和31.8%，而儿童和成人对LEV和TET的耐药菌株分离频率则低得多，分别为9.1%和4.5%以及18.4%和4.1%。未观察到对AMX耐药的菌株。从波兰原发性感染患者中分离出的[细菌名称]菌株对CLR和MTZ表现出较高水平的抗生素耐药性（>30%）。
