Kudłacik-Kramarczyk Sonia, Głąb Magdalena, Drabczyk Anna, Kordyka Aleksandra, Godzierz Marcin, Wróbel Paweł S, Krzan Marcel, Uthayakumar Marimuthu, Kędzierska Magdalena, Tyliszczak Bożena
Department of Materials Science, Faculty of Materials Engineering and Physics, Cracow University of Technology, 37 Jana Pawła II Av., 31-864 Krakow, Poland.
Centre of Polymer and Carbon Materials, Polish Academy of Sciences; 34 M. Curie-Skłodowskiej St., 41-819 Zabrze, Poland.
Materials (Basel). 2021 Oct 6;14(19):5832. doi: 10.3390/ma14195832.
In recent years, many investigations on the development of innovative dressing materials with potential applications, e.g., for cytostatics delivery, have been performed. One of the most promising carriers is albumin, which tends to accumulate near cancer cells. Here, chitosan-based hydrogels containing albumin spheres and juice, designed for the treatment of skin cancers or burn wounds resulting from radiotherapy, were developed. The presence of albumin in hydrogel matrices was confirmed via Fourier transform infrared (FT-IR) and Raman spectroscopy. Albumin spheres were clearly visible in microscopic images. It was proved that the introduction of albumin into hydrogels resulted in their increased resistance to the tensile load, i.e., approximately 30% more force was needed to break such materials. Modified hydrogels showed approximately 10% more swelling ability. All hydrogels were characterized by hydrophilicity (contact angles were <90°) which may support the regeneration of epithelial cells and non-cytotoxicity towards murine fibroblasts L929 and released juice more effectively in an acidic environment than in a neutral one wherein spheres introduced into the hydrogel matrix extended the release time. Thus, the developed materials, due to their chemical composition and physicochemical properties, constitute promising materials with great application potential for biomedical purposes.
近年来，人们对具有潜在应用价值的创新敷料材料开展了许多研究，例如用于细胞抑制剂递送。最有前景的载体之一是白蛋白，它倾向于在癌细胞附近积聚。在此，开发了一种基于壳聚糖的水凝胶，其中含有白蛋白球体和果汁，用于治疗皮肤癌或放疗引起的烧伤创面。通过傅里叶变换红外（FT-IR）光谱和拉曼光谱证实了水凝胶基质中白蛋白的存在。在显微镜图像中可以清楚地看到白蛋白球体。结果表明，将白蛋白引入水凝胶中会使其抗拉伸载荷能力增强，即破坏此类材料所需的力大约增加30%。改性水凝胶的溶胀能力提高了约10%。所有水凝胶均具有亲水性（接触角<90°），这可能有助于上皮细胞的再生，并且对小鼠成纤维细胞L929无细胞毒性，在酸性环境中比在中性环境中更有效地释放果汁，其中引入水凝胶基质的球体延长了释放时间。因此，所开发的材料因其化学成分和物理化学性质，构成了具有巨大生物医学应用潜力的有前景的材料。
