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用于识别的在1,1'-羰基二咪唑功能化表面上的靶向DNA互补作用

Targeted DNA complementation on a 1,1'-carbonyldiimidazole-functionalized surface for identifying .

作者信息

Xu Shu, Xue Yu, Guo Fengyan, Xu Miaomiao, Gopinath Subash C B, Mao Xiaohui

机构信息

Department of Tuberculosis Complications, Xi'an Chest Hospital, East Section of Aerospace Avenue, Chang'an District, Xi'an, 710100 Shaanxi China.

2School of Bioprocess Engineering, Universiti Malaysia Perlis, 02600 Arau, Malaysia.

出版信息

3 Biotech. 2020 May;10(5):227. doi: 10.1007/s13205-020-02216-2. Epub 2020 May 2.

DOI:10.1007/s13205-020-02216-2
PMID:32373419
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7196115/
Abstract

Herein, a rapid and sensitive current-volt measurement was developed for identifying the IS6110 DNA sequence to diagnose (TB). An aminated capture probe was immobilized on a 1,1'-carbonyldiimidazole-functionalized interdigitated electrode (IDE) silica substrate, and the target sequence was detected by complementation. It was found that all tested concentrations displayed a higher response in current changes than the control, and the limit of detection was 10 fM. The sensitivity ranged from 1 to 10 fM. The control sequences with single-, triple-mismatch and noncomplementary sequences showed great discrimination. This rapid and easy DNA detection method helps to identify for early-stage diagnosis of TB.

摘要

在此，开发了一种快速灵敏的电流-电压测量方法，用于鉴定IS6110 DNA序列以诊断结核病（TB）。将胺化捕获探针固定在1,1'-羰基二咪唑功能化的叉指电极（IDE）二氧化硅基板上，并通过互补作用检测目标序列。结果发现，所有测试浓度在电流变化方面均显示出比对照更高的响应，检测限为10 fM。灵敏度范围为1至10 fM。具有单错配、三错配和非互补序列的对照序列显示出很好的区分度。这种快速简便的DNA检测方法有助于结核病的早期诊断鉴定。