Pahlavan Yasamin, Samadi Naser, Ansarin Khalil, Khabbazi Alireza
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Connective Tissue Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Adv Pharm Bull. 2020 Jun;10(2):278-283. doi: 10.34172/apb.2020.033. Epub 2020 Feb 18.
Survivin is critical for proliferation, maturation, homeostasis and differentiation of effector and memory lymphocytes. In this study the baculoviral inhibitors of apoptosis proteins (IAPs) repeat containing 5 (BIRC5) mRNA, survivin, and phosphorylated survivin expression were evaluated in peripheral blood mononuclear cells (PBMCs), and plasma of patients with Behcet's disease (BD). In this study, 26 Iranian Azari patients diagnosed with BD and 30 healthy controls were recruited. Total RNA was extracted from PBMCs. The expression level of survivin was measured by quantitative real-time polymerase chain reaction (PCR). Survivin plasma levels were measured using survivin Enzyme-linked immunosorbent assays. Also, western blotting analysis was performed to measure phosphorylated-survivin and survivin levels in PBMCs and plasma of patients with BD. In a pilot study, we showed that BIRC5 gene expression increased in BD patients compared with healthy controls (<0.05). Western blotting analysis indicated that there was an increase in phosphorylated survivin expression in PBMCs of BD patients. Our data from western blot analysis showed survivin level in plasma samples of BD patients was similar to healthy controls. No significant differences were observed between plasma survivin levels in the BD patients compared with control group (>0.05). The expression of phosphorylated survivin at Thr34 in PBMCs of BD patients with active disease was increased. Plasma phosphorylated survivin levels in having BD patients were also downregulated compared to healthy individuals. Analysis of PBMCs indicated increasing expression level of phosphorylated survivin in PBMCs of BD patients. There was also a downregulation in phosphorylated survivin levels in plasma of BD patients.
生存素对于效应淋巴细胞和记忆淋巴细胞的增殖、成熟、稳态及分化至关重要。在本研究中，对白塞病（BD）患者的外周血单个核细胞（PBMC）及血浆中的含杆状病毒凋亡抑制蛋白（IAPs）重复序列5（BIRC5）mRNA、生存素以及磷酸化生存素表达进行了评估。本研究招募了26名被诊断为BD的伊朗阿扎里患者及30名健康对照者。从PBMC中提取总RNA。通过定量实时聚合酶链反应（PCR）测定生存素的表达水平。使用生存素酶联免疫吸附测定法测量血浆中生存素水平。此外，还进行了蛋白质印迹分析以测定BD患者PBMC及血浆中磷酸化生存素和生存素水平。在一项初步研究中，我们发现与健康对照相比，BD患者中BIRC5基因表达增加（<0.05）。蛋白质印迹分析表明，BD患者PBMC中磷酸化生存素表达增加。我们蛋白质印迹分析的数据显示，BD患者血浆样本中的生存素水平与健康对照相似。与对照组相比，BD患者血浆生存素水平未观察到显著差异（>0.05）。活动性疾病的BD患者PBMC中苏氨酸34位点磷酸化生存素的表达增加。与健康个体相比，BD患者血浆中磷酸化生存素水平也下调。PBMC分析表明，BD患者PBMC中磷酸化生存素表达水平增加。BD患者血浆中磷酸化生存素水平也有下调。
