Cheung Jimmy Ka-Wai, Li King-Kit, Zhou Lei, To Chi-Ho, Lam Thomas Chuen
Laboratory of Experimental Optometry, Centre for Myopia Research, School of Optometry, The Hong Kong Polytechnic University, Hong Kong Special Administractive Region of China.
Ocular Proteomics Laboratory, Singapore Eye Research Institute, Singapore.
Data Brief. 2020 Apr 18;30:105576. doi: 10.1016/j.dib.2020.105576. eCollection 2020 Jun.
Myopia is the most common refractive error which is estimated to affect half the population of the world by 2050. It has been suggested that it could be determined by multiple factors such as environmental and genetic, but the mechanism behind the cause of myopia is still yet to be identified. Vitreous humor (VH) is a transparent gelatin-like substance that takes up to 80% of the volume of the eye, making it the largest component of the eye. Although VH is the main contributor to axial elongation of the eye including normal eye growth (emmetropization) and myopia, the diluted nature of VH (made up of 99% of water) made it difficult for less abundant molecules to be identified and therefore often overlooked. Using the more sensitive label-free mass spectrometry approach with data-independent acquisition (SWATH-MS), we established a comprehensive VH proteome library in chick animal model and quantified possible protein biomarkers that are responsible for the axial elongation during emmetropization (7, 14, 21, 28 days after hatching, = 48 eyes). Raw data files for both information-dependent acquisition (IDA) and data-independent acquisition (SWATH-MS) were uploaded on PeptideAtlas for public access (http://www.peptideatlas.org/PASS/PASS01258).
近视是最常见的屈光不正，据估计到2050年将影响全球一半的人口。有人认为它可能由多种因素决定，如环境和遗传因素，但近视成因背后的机制仍有待确定。玻璃体液（VH）是一种透明的凝胶状物质，占眼球体积的80%，是眼球最大的组成部分。尽管玻璃体液是眼球轴向伸长的主要因素，包括正常眼球生长（正视化）和近视，但玻璃体液的稀释性质（99%由水组成）使得含量较少的分子难以被识别，因此常常被忽视。我们使用更灵敏的无标记质谱方法和数据非依赖采集（SWATH-MS），在雏鸡动物模型中建立了一个全面的玻璃体液蛋白质组文库，并对可能在正视化过程中（孵化后7、14、21、28天，n = 48只眼）负责轴向伸长的蛋白质生物标志物进行了定量分析。信息依赖采集（IDA）和数据非依赖采集（SWATH-MS）的原始数据文件已上传至PeptideAtlas以供公众访问（http://www.peptideatlas.org/PASS/PASS01258）。
